In vitro selected oligonucleotides as receptors in binding assays

被引:11
作者
Ito, Y [1 ]
Kawazoe, N
Imanishi, Y
机构
[1] Univ Tokushima, Fac Engn, Dept Biol Sci & Technol, Tokushima 7708506, Japan
[2] Japan Sci & Technol Corp, PRESTO, Kyoto 6190237, Japan
[3] NAIST, Grad Sch Mat Sci, Ikoma 6300101, Japan
来源
METHODS-A COMPANION TO METHODS IN ENZYMOLOGY | 2000年 / 22卷 / 01期
关键词
D O I
10.1006/meth.2000.1041
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This paper describes a new binding assay that uses oligodeoxyribonucleotides (DNAs) obtained by the in vitro selection method instead of antibodies. DNAs that specifically bound to a target molecule were selected, labeled with some probes, and then used to detect the target molecule by staining or competitive binding assay. (C) 2000 Academic Press.
引用
收藏
页码:107 / 114
页数:8
相关论文
共 27 条
[1]   In vivo imaging of inflammation using an aptamer inhibitor of human neutrophil elastase [J].
Charlton, J ;
Sennello, J ;
Smith, D .
CHEMISTRY & BIOLOGY, 1997, 4 (11) :809-816
[2]   Detecting immobilized protein kinase C isozymes with RNA aptamers [J].
Conrad, R ;
Ellington, AD .
ANALYTICAL BIOCHEMISTRY, 1996, 242 (02) :261-265
[3]   Use of a high affinity DNA ligand in flow cytometry [J].
Davis, KA ;
Abrams, B ;
Lin, Y ;
Jayasena, SD .
NUCLEIC ACIDS RESEARCH, 1996, 24 (04) :702-706
[4]   NEW URIDINE DERIVATIVES FOR SYSTEMATIC EVOLUTION OF RNA LIGANDS BY EXPONENTIAL ENRICHMENT [J].
DEWEY, TM ;
MUNDT, AA ;
CROUCH, GJ ;
ZYZNIEWSKI, MC ;
EATON, BE .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (32) :8474-8475
[5]   An enzyme-linked oligonucleotide assay [J].
Drolet, DW ;
MoonMcDermott, L ;
Romig, TS .
NATURE BIOTECHNOLOGY, 1996, 14 (08) :1021-1025
[6]   SELECTION INVITRO OF SINGLE-STRANDED-DNA MOLECULES THAT FOLD INTO SPECIFIC LIGAND-BINDING STRUCTURES [J].
ELLINGTON, AD ;
SZOSTAK, JW .
NATURE, 1992, 355 (6363) :850-852
[7]   INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].
ELLINGTON, AD ;
SZOSTAK, JW .
NATURE, 1990, 346 (6287) :818-822
[8]   OLIGONUCLEOTIDES AS RESEARCH, DIAGNOSTIC, AND THERAPEUTIC AGENTS [J].
GOLD, L .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (23) :13581-13584
[9]   Competitive binding assay for thyroxine using in vitro selected oligonucleotides [J].
Ito, Y ;
Fujita, S ;
Kawazoe, N ;
Imanishi, Y .
ANALYTICAL CHEMISTRY, 1998, 70 (16) :3510-3512
[10]   Modified nucleic acid for systematic evolution of RNA ligands by exponential enrichment [J].
Ito, Y ;
Teramoto, N ;
Kawazoe, N ;
Inada, K ;
Imanishi, Y .
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, 1998, 13 (02) :114-123