Simultaneous analysis of cyclophosphamide, doxorubicin and doxorubicinol by liquid chromatography coupled to tandem mass spectrometry

被引：54

作者：

DiFrancesco, Robin

Griggs, Jennifer J.

Donnelly, Julie

DiCenzo, Robert

机构：

[1] SUNY Buffalo, Dept Pharm Practice, Buffalo, NY 14260 USA

[2] Univ Michigan, Dept Med Hematol Oncol, Ann Arbor, MI 48109 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 852卷 / 1-2期

关键词：

anthracyclines; pharmacokinetics; obesity; assay;

D O I：

10.1016/j.jchromb.2007.02.033

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the simultaneous determination of cyclophosphamide (CP), doxombicin (dox), and doxorubicinol (dol) was developed and validated to analyze 400 mu L of plasma from patients receiving chemotherapeutic treatment with CP and dox. Final calibration ranges for the analytes were 0.440-60.0 mu g/mL for cyclophosphamide, 7.20-984 ng/mL for dox and 3.04-104 ng/mL for dol. The samples were prepared using solid phase extraction and analyzed using a gradient separation over a Waters Symmetry (R) C18, 2.1 by 30 mm (Milford, MA) column. Detection was achieved in positive mixed reaction monitoring mode on a triple quadrupole mass spectrometer. (c) 2007 Elsevier B.V. All rights reserved.

引用

收藏

页码：545 / 553

页数：9

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