Determination of tyrosine through a FIA-direct chemiluminescence procedure

被引:53
作者
Alonso, MCS
Zamora, LL
Calatayud, JM [1 ]
机构
[1] Univ Valencia, Dept Quim Analit, Fac Ciencias Quim, E-46100 Valencia, Spain
[2] Univ Cardenal Herrera, Dept Quim Bioquim & Biol Mol, CEU, Valencia 46113, Spain
关键词
tyrosine; chemiluminescence; continuous-flow; pharmaceuticals;
D O I
10.1016/S0039-9140(03)00099-7
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new FI-direct chemiluminescence method is proposed for the determination of tyrosine, based on the oxidation of the amino acid by K3Fe(CN)(6) in potassium hydroxide medium, at room temperature and enhanced by the presence of beta-cyclodextrin and formic acid. The dynamic range was linear over the range 1.0 - 10.0 mg 1(-1) A large study of the influence of foreign compounds was performed, including amino acids; and, the method showed high selectivity. The reproducibility between days resulted in a rsd (in slope%) of 4.8 and the repeatability with a rsd (n = 50, 10.0 mg I of 3.1%, the LOD (s/n = 3) was 50 mug 1(-1) and sample throughput 98 h(-1). (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:369 / 376
页数:8
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