Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy

被引:123
作者
Prabhat, Prashant
Gan, Zhuo
Chao, Jerry
Ram, Sripad
Vaccaro, Carlos
Gibbons, Steven
Ober, Raimund J. [1 ]
Ward, E. Sally
机构
[1] Univ Texas, SW Med Ctr, Dept Immunol, Dallas, TX 75390 USA
[2] Univ Texas, Dept Elect Engn, Richardson, TX 75080 USA
关键词
immunoglobulin G; multifocal plane imaging; neonatal Fc receptor; sorting endosome; total internal reflection;
D O I
10.1073/pnas.0700337104
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The intracellular events on the recycling pathway that lead from sorting endosomes to exocytosis at the plasma membrane are central to cellular function. However, despite intensive study, these processes are poorly characterized in spatial and dynamic terms. The primary reason for this is that, to date, it has not been possible to visualize rapidly moving intracellular compartments in three dimensions in cells. Here, we use a recently developed imaging setup in which multiple planes can be simultaneously imaged within the cell in conjunction with visualization of the plasma membrane plane by using total internal reflection fluorescence microscopy. This has allowed us to track and characterize intracellular events on the recycling pathway that lead to exocytosis of the MHC Class I-related receptor, FcRn. We observe both direct delivery of tubular and vesicular transport containers (TCs) from sorting endosomes to exocytic sites at the plasma membrane, and indirect pathways in which TCs that are not in proximity to sorting endosomes undergo exocytosis. TCs can also interact with different sorting endosomes before exocytosis. Our data provide insight into the intracellular events that precede exocytic fusion.
引用
收藏
页码:5889 / 5894
页数:6
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