HPLC-FLU detection of cortisol distribution in human hair

被引：86

作者：

Gao, Wei ^{[1
]}

Xie, Qiaozhen ^{[1
]}

Jin, Jing ^{[1
,2
]}

Qiao, Ting ^{[1
]}

Wang, Haiyang ^{[1
]}

Chen, Liqin ^{[1
]}

Deng, Huihua ^{[1
]}

Lu, Zuhong ^{[1
,2
]}

机构：

[1] Southeast Univ, Res Ctr Learning Sci, Key Lab Child Dev & Learning Sci, Minist Educ, Nanjing 210096, Peoples R China

[2] Southeast Univ, Sch Biol Sci & Med Engn, Chien Shiung Wu Lab, Nanjing 210096, Peoples R China

来源：

CLINICAL BIOCHEMISTRY | 2010年 / 43卷 / 7-8期

关键词：

Hair; Cortisol; High performance liquid chromatography; Distribution; SALIVARY CORTISOL; STRESS;

D O I：

10.1016/j.clinbiochem.2010.01.014

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objective: Recently cortisol level in hair is supposed to be a good biomarker of chronic stress. We had developed a new method based on HPLC-FLU for identification of cortisol in human hair and measured cortisol distribution of each 1-cm segment along hair shaft. Design and methods: Hair sample was pulverized and incubated in 0.1 M HCl and extracted with ethyl acetate. The extraction was derived with sulfuric acid. The derivative was extracted with SPE C18 column. Results: The detection limit was 1 pg/mg, the intraday and interday precisions were 7.3% and 8.5%, and average recovery was 102.5 +/- 13.0%. Cortisol concentrations of 32 humans' hair ranged from 3.28 to 24.83 pg/mg. There was a distinct decline in the cortisol of 1-cm segment along hair shaft. Conclusions: This method showed that the precision was matched with mass spectroscopy. The cortisol in 1-cm hair segment sensitively reflects a retrospective calendar over 1 month. (C) 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

引用

页码：677 / 682

页数：6

共 14 条

[1] Identification of ten corticosteroids in human hair by liquid chromatography-ionspray mass spectrometry [J].