Changes in the Lactobacillus community during Ricotta forte cheese natural fermentation

被引:62
作者
Baruzzi, F
Morea, M
Matarante, A
Cocconcelli, PS
机构
[1] CNR, Ist Tossine & Micotossine Parassiti Vegetali, I-70125 Bari, Italy
[2] Univ Cattolica Sacro Cuore, Ist Microbiol, Piacenza, Italy
[3] Univ Cattolica Sacro Cuore, Ctr Ric Biotecnol, Cremona, Italy
关键词
D O I
10.1046/j.1365-2672.2000.01183.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The loss of microbial biodiversity due to the increase in large-scale industrial processes led to the study of the natural microflora present in a typical little known dairy product. The community of lactobacilli was studied in order to understand the natural fermentation of Ricotta forte cheese. The combined use of RAPD analysis, 16S rDNA sequencing and physiological tests allowed 33 different strains belonging to 10 species of Lactobacillus to be characterized. RAPD analysis revealed the heterogeneity of both the Lact. kefiri and Lact. paracasei species. The sequence analysis of the large 16S/23S rRNA spacer region enabled Lact. plantarum to be distinguished from Lact. paraplantarum, two closely related species belonging to the Lact. plantarum group. The recovery of strains endowed with interesting physiological characteristics, such as strong stress resistance, could improve technological and/or organoleptic characteristics of Ricotta forte cheese and other fermented foods.
引用
收藏
页码:807 / 814
页数:8
相关论文
共 26 条
  • [1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
    Altschul, SF
    Madden, TL
    Schaffer, AA
    Zhang, JH
    Zhang, Z
    Miller, W
    Lipman, DJ
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
  • [2] Identification and characterization of proteolytic activity of Enterococcus spp. isolated from milk and Roncal and Idiazabal cheese
    Arizcun, C
    Barcina, Y
    Torre, P
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1997, 38 (01) : 17 - 24
  • [3] Berthier F, 1998, FEMS MICROBIOL LETT, V161, P97, DOI [10.1016/S0378-1097(98)00055-X, 10.1111/j.1574-6968.1998.tb12934.x]
  • [4] SPECTROPHOTOMETRIC ASSAY USING ORTHO-PHTHALDIALDEHYDE FOR DETERMINATION OF PROTEOLYSIS IN MILK AND ISOLATED MILK-PROTEINS
    CHURCH, FC
    SWAISGOOD, HE
    PORTER, DH
    CATIGNANI, GL
    [J]. JOURNAL OF DAIRY SCIENCE, 1983, 66 (06) : 1219 - 1227
  • [5] DEVELOPMENT OF RAPD PROTOCOL FOR TYPING OF STRAINS OF LACTIC-ACID BACTERIA AND ENTEROCOCCI
    COCCONCELLI, PS
    PORRO, D
    GALANDINI, S
    SENINI, L
    [J]. LETTERS IN APPLIED MICROBIOLOGY, 1995, 21 (06) : 376 - 379
  • [6] Use of RAPD and 16S rDNA sequencing for the study of Lactobacillus population dynamics in natural whey culture
    Cocconcelli, PS
    Parisi, MG
    Senini, L
    Bottazzi, V
    [J]. LETTERS IN APPLIED MICROBIOLOGY, 1997, 25 (01) : 8 - 12
  • [7] Corroler D, 1998, APPL ENVIRON MICROB, V64, P4729
  • [8] DIVERSITY OF CELL-ENVELOPE PROTEINASE SPECIFICITY AMONG STRAINS OF LACTOCOCCUS-LACTIS AND ITS RELATIONSHIP TO CHARGE CHARACTERISTICS OF THE SUBSTRATE-BINDING REGION
    EXTERKATE, FA
    ALTING, AC
    BRUINENBERG, PG
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (11) : 3640 - 3647
  • [9] Fitzsimons NA, 1999, APPL ENVIRON MICROB, V65, P3418
  • [10] BIOCHEMICAL AND MICROBIOLOGICAL CHARACTERISTICS OF ARTISANAL HARD GOATS CHEESE
    FONTECHA, J
    PELAEZ, C
    JUAREZ, M
    REQUENA, T
    GOMEZ, C
    RAMOS, M
    [J]. JOURNAL OF DAIRY SCIENCE, 1990, 73 (05) : 1150 - 1157