Intense correlation between protein-conjugated acrolein and primary Sjogren's syndrome

被引：28

作者：

Higashi, Kyohei ^{[1
,2
]}

Yoshida, Madoka ^{[1
,2
]}

Igarashi, Atsuko ^{[3
]}

Ito, Kayoko ^{[4
]}

Wada, Yoko ^{[3
]}

Murakami, Shuichi ^{[3
]}

Kobayashi, Daisuke ^{[3
]}

Nakano, Masaaki ^{[5
]}

Sohda, Miwa ^{[3
]}

Nakajima, Takako ^{[4
]}

Narita, Ichiei ^{[3
]}

Toida, Toshihiko ^{[1
]}

Kashiwagi, Keiko ^{[6
]}

Igarashi, Kazuei ^{[1
,2
]}

机构：

[1] Chiba Univ, Grad Sch Pharmaceut Sci, Chuo Ku, Chiba 2608675, Japan

[2] Chiba Univ, Amine Pharma Res Inst, Chuo Ku, Chiba 2600856, Japan

[3] Niigata Univ, Grad Sch Med & Dent Sci, Chuo Ku, Niigata 9518514, Japan

[4] Niigata Univ Med & Dent Hosp, Chuo Ku, Niigata 9518510, Japan

[5] Niigata Univ, Fac Med, Sch Hlth Sci, Chuo Ku, Niigata 9518518, Japan

[6] Chiba Inst Sci, Fac Pharm, Chiba 2880025, Japan

来源：

CLINICA CHIMICA ACTA | 2010年 / 411卷 / 5-6期

关键词：

Albumin-conjugated acrolein; Saliva; Flow rate; Primary Sjogren's syndrome; SALIVARY-GLANDS; RENAL-FAILURE; AMINE OXIDASE; MATRIX-METALLOPROTEINASE-9; IDENTIFICATION; ASSOCIATION; INHIBITOR; SYMPTOMS; INCREASE; DAMAGE;

D O I：

10.1016/j.cca.2009.11.032

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: We recently found that an increased plasma concentration of protein-conjugated acrolein is a good biomarker for stroke. Therefore we determine whether the concentration of protein-conjugated acrolein is increased in saliva from patients with primary Sjogren's syndrome. Methods: Stimulated whole-mixed saliva was collected from 10 patients and 13 control subjects. The concentration of protein-conjugated acrolein in saliva and plasma was measured by either Western blotting or enzyme-linked immunosorbent assay. Results: The concentration of protein-conjugated acrolein, especially albumin-conjugated acrolein, was greatly increased in saliva from patients with primary Sjogren's syndrome (p<0.001). The concentration of protein-conjugated acrolein was inversely correlated with the flow rate of saliva. Conclusion: The results indicate that the concentration of protein-conjugated acrolein, a marker of cell or tissue damage, in saliva is well correlated with seriousness of primary Sjogren's syndrome. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：359 / 363

页数：5

共 25 条

[1] Increase in the ratio of matrix metalloproteinase-9 to tissue inhibitor of metalloproteinase-1 in saliva from patients with primary Sjogren's syndrome [J].

Asatsuma, M ;

Ito, S ;

Watanabe, M ;

Takeishi, H ;

Nomura, S ;

Wada, Y ;

Nakano, M ;

Gejyo, F ;

Igarashi, A .

CLINICA CHIMICA ACTA, 2004, 345 (1-2) :99-104

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] Indicators of salivary gland inflammation in primary Sjogren's syndrome [J].

Cuida, M ;

Halse, AK ;

Johannessen, AC ;

Tynning, T ;

Jonsson, R .

EUROPEAN JOURNAL OF ORAL SCIENCES, 1997, 105 (03) :228-233

[4] Proteome analysis of whole saliva: A new tool for rheumatic diseases - the example of Sjogren's syndrome [J].

Giusti, Laura ;

Baldini, Chiara ;

Bazzichi, Laura ;

Ciregia, Federica ;

Tonazzini, Ilaria ;

Mascia, Giovanni ;

Giannaccini, Gino ;

Bombardier, Stefano ;

Lucacchini, Antonio .

PROTEOMICS, 2007, 7 (10) :1634-1643

[5] A novel and simple immunocapture assay for determination of gelatinase-B (MMP-9) activities in biological fluids: Saliva from patients with Sjogren's syndrome contain increased latent and active gelatinase-B levels [J].

Hanemaaijer, R ;

Visser, H ;

Konttinen, YT ;

Koolwijk, P ;

Verheijen, JH .

MATRIX BIOLOGY, 1998, 17 (8-9) :657-665

[6] Weak association between subjective symptoms of and objective testing for dry eyes and dry mouth: results from a population based study [J].

Hay, EM ;

Thomas, E ;

Pal, B ;

Hajeer, A ;

Chambers, H ;

Silman, AJ .

ANNALS OF THE RHEUMATIC DISEASES, 1998, 57 (01) :20-24

[7] Reactive oxygen species, cell signaling, and cell injury [J].

Hensley, K ;

Robinson, KA ;

Gabbita, SP ;

Salsman, S ;

Floyd, RA .

FREE RADICAL BIOLOGY AND MEDICINE, 2000, 28 (10) :1456-1462

[8] Large-scale identification of proteins in human salivary proteome by liquid chromatography/mass spectrometry and two-dimensional gel electrophoresis-mass spectrometry [J].

Hu, S ;

Xie, YM ;

Ramachandran, P ;

Loo, RRO ;

Li, Y ;

Loo, JA ;

Wong, DT .

PROTEOMICS, 2005, 5 (06) :1714-1728

[9] Salivary proteomic and genomic biomarkers for primary Sjogren's syndrome [J].

Hu, Shen ;

Wang, Jianghua ;

Meijer, Jiska ;

Leong, Sonya ;

Xie, Yongming ;

Yu, Tianwei ;

Zhou, Hui ;

Henry, Sharon ;

Vissink, Arjan ;

Pijpe, Justin ;

Kallenberg, Cees ;

Elashoff, David ;

Loo, Joseph A. ;

Wong, David T. .

ARTHRITIS AND RHEUMATISM, 2007, 56 (11) :3588-3600

[10] Modulation of cellular function by polyamines [J].

Iarashi, Kazuei ;

Kashiwagi, Keiko .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2010, 42 (01) :39-51

← 1 2 3 →