Involvement of acetosyringone in plant-pathogen recognition

被引:51
作者
Baker, CJ [1 ]
Mock, NM
Whitaker, BD
Roberts, DP
Rice, CP
Deahl, KL
Aver'yanov, AA
机构
[1] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA
[2] USDA ARS, Produce Qual & Safety Lab, Beltsville, MD 20705 USA
[3] USDA ARS, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA
[4] USDA ARS, Environm Qual Lab, Beltsville, MD 20705 USA
[5] USDA ARS, Vegetable Lab, Beltsville, MD 20705 USA
[6] Phytopathol Res Inst, B Vyazemy 143050, Moscow Region, Russia
关键词
oxidative burst; oxygen uptake; reactive oxygen; hydrogen peroxide; Pseudomonas syringae; Pseudomonas tabaci; Nicotiana tabacum; phenolics; apoplast;
D O I
10.1016/j.bbrc.2004.12.153
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study, acetosyringone was identified as one of the major extracellular phenolics in tobacco suspension cells and was shown to have bioactive properties that influence early events in plant-bacterial pathogenesis. In our model system, tobacco cell suspensions treated with bacterial isolate Pseudomonas syringae WT (HR+) undergo a resistant interaction characterized by a burst in oxygen uptake several hours after inoculation. When the extracellular concentration of acetosyringone in tobacco cell suspensions was supplemented with exogenous acetosyringone, the burst in oxygen uptake occurred as much as 1.5 h earlier. The exogenous acetosyringone had no effect on tobacco suspensions undergoing susceptible interactions with Pseudomonas tabaci or a non-resistant interaction with a near-isogenic mutant derivative of isolate P. syringae WT (HR+). Resistant interactions with isolate P. syringae WT (HR+) also produce an oxidative burst which oxidizes the extracellular acetosyringone. This study demonstrates that acetosyringone, and likely other extracellular phenolics, may have bioactive characteristics that can influence plant-bacterial pathogenesis. Published by Elsevier Inc.
引用
收藏
页码:130 / 136
页数:7
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