Technology for high-throughput screens: the present and future using zebrafish

被引：87

作者：

Love, DR

Pichler, FB

Dodd, A

Copp, BR

Greenwood, DR

机构：

[1] Univ Auckland, Sch Biol Sci, Auckland 1001, New Zealand

[2] Univ Auckland, Dept Chem, Auckland 1001, New Zealand

[3] Gene Technol, HortRes, Mt Albert Res Ctr, Auckland 1003, New Zealand

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2004年 / 15卷 / 06期

关键词：

D O I：

10.1016/j.copbio.2004.09.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The zebrafish is a popular vertebrate model organism with similar organ systems and gene sequences to humans. Zebrafish embryos are optically transparent enabling organ visualisation, which can be complemented with gene expression analysis at the transcript and protein levels. Furthermore, zebrafish can be treated with small molecules and drugs in a microtitre plate format for high-throughput analysis and for the identification and validation of drugs. High-throughput methodologies for use in zebrafish include phenotype-based visualisation, transcript studies using low-density DNA microarrays and proteomic analysis. These technologies offer significant whole-organism biological value in the drug discovery and drug development pipeline.

引用

收藏

页码：564 / 571

页数：8

相关论文

共 53 条

[1] A need for a 'whole-istic functional genomics' approach in complex human diseases: arthritis [J].

Amin, AR .

ARTHRITIS RESEARCH & THERAPY, 2003, 5 (02) :76-79

[2] Identification of modulators of TRAIL-induced apoptosis via RNAi-based phenotypic screening [J].

Aza-Blanc, P ;

Cooper, CL ;

Wagner, K ;

Batalov, S ;

Deveraux, QL ;

Cooke, MP .

MOLECULAR CELL, 2003, 12 (03) :627-637

[3] An automated high performance capillary liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometer for high-throughput proteomics [J].

Belov, ME ;

Anderson, GA ;

Wingerd, MA ;

Udseth, HR ;

Tang, KQ ;

Prior, DC ;

Swanson, KR ;

Buschbach, MA ;

Strittmatter, EF ;

Moore, RJ ;

Smith, RD .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2004, 15 (02) :212-232

[4] A large-scale RNAi screen in human cells identifies new components of the p53 pathway [J].

Berns, K ;

Hijmans, EM ;

Mullenders, J ;

Brummelkamp, TR ;

Velds, A ;

Heimerikx, M ;

Kerkhoven, RM ;

Madiredjo, M ;

Nijkamp, W ;

Weigelt, B ;

Agami, R ;

Ge, W ;

Cavet, G ;

Linsley, PS ;

Beijersbergen, RL ;

Bernards, R .

NATURE, 2004, 428 (6981) :431-437

[5] Visualizing the distribution and transport of mRNAs in living cells [J].

Bratu, DP ;

Cha, BJ ;

Mhlanga, MM ;

Kramer, FR ;

Tyagi, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (23) :13308-13313

[6] Minimum information about a microarray experiment (MIAME) - toward standards for microarray data [J].

Brazma, A ;

Hingamp, P ;

Quackenbush, J ;

Sherlock, G ;

Spellman, P ;

Stoeckert, C ;

Aach, J ;

Ansorge, W ;

Ball, CA ;

Causton, HC ;

Gaasterland, T ;

Glenisson, P ;

Holstege, FCP ;

Kim, IF ;

Markowitz, V ;

Matese, JC ;

Parkinson, H ;

Robinson, A ;

Sarkans, U ;

Schulze-Kremer, S ;

Stewart, J ;

Taylor, R ;

Vilo, J ;

Vingron, M .

NATURE GENETICS, 2001, 29 (04) :365-371

[7] High-throughput imaging of brain gene expression [J].

Brown, VM ;

Ossadtchi, A ;

Khan, AH ;

Cherry, SR ;

Leahy, RM ;

Smith, DJ .

GENOME RESEARCH, 2002, 12 (02) :244-254

[8] Interpretation of mass spectrometry data for high-throughput proteomics [J].

Chamrad, DC ;

Koerting, G ;

Gobom, J ;

Thiele, H ;

Klose, J ;

Meyer, HE ;

Blueggel, M .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2003, 376 (07) :1014-1022

[9] Short interfering RNA-mediated gene targeting in the zebrafish [J].

Dodd, A ;

Chambers, SP ;

Love, DR .

FEBS LETTERS, 2004, 561 (1-3) :89-93

[10] High-throughput proteomics: A flexible and efficient pipeline for protein production [J].

Doyle, SA ;

Murphy, MB ;

Massi, JM ;

Richardson, PM .

JOURNAL OF PROTEOME RESEARCH, 2002, 1 (06) :531-536

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