Matrix-implanted laser desorption/ionization mass spectrometry

被引：19

作者：

Novikov, A

Caroff, M

Della-Negra, S

Lebeyec, Y

Pautrat, M

Schultz, JA

Tempez, A

Wang, HYJ

Jackson, SN

Woods, AS

机构：

[1] NIDA, IRP, Baltimore, MD 21224 USA

[2] CNRS, IN2P3, Inst Nucl Phys, F-91406 Orsay, France

[3] Univ Paris 11, CNRS, Equipe Endotoxines, UMR 8619,IBBMC, F-91405 Orsay, France

[4] Ionwerks Inc, Houston, TX 77030 USA

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 24期

关键词：

D O I：

10.1021/ac049123i

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The implantation of low-velocity massive gold clusters is shown to be a method of choice for homogeneous incorporation of a metallic matrix into the near-surface region of a solid biopolymer for subsequent laser desorption/ionization (LDI) MS analysis. Matrix implanted (MI)LDI spectra from cluster-implanted pure test peptide or tissue exhibit molecular ion peaks similar to those observed by matrix-assisted LDI. Moreover, the ion emission is very reproducible from any spot on the surface of these test samples. MILDI promises to be a powerful technique for mass spectrometric analysis of native biological samples as demonstrated by the first results on rat brain tissues.

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收藏

页码：7288 / 7293

页数：6

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