VirB/D4-dependent protein translocation from Agrobacterium into plant cells

被引：299

作者：

Vergunst, AC ^{[1
]}

Schrammeijer, B ^{[1
]}

den Dulk-Ras, A ^{[1
]}

de Vlaam, CMT ^{[1
]}

Regensburg-Tuïnk, TJG ^{[1
]}

Hooykaas, PJJ ^{[1
]}

机构：

[1] Leiden State Univ, Inst Mol Plant Sci, Clusius Lab, NL-2333 AL Leiden, Netherlands

来源：

SCIENCE | 2000年 / 290卷 / 5493期

关键词：

D O I：

10.1126/science.290.5493.979

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Agrobacterium VirB/D4 transport system mediates the transfer of a nucleoprotein T complex into plant cells, Leading to crown gall disease. In addition, several Virulence proteins must somehow be transported to fulfill a function in planta. Here, we used fusions between Cre recombinase and VirE2 or VirF to directly demonstrate protein translocation into plant cells. Transport of the proteins was monitored by a Cre-mediated in planta recombination event resulting in a selectable phenotype and depended on the VirB/D4 transport system but did not require transferred DNA.

引用

页码：979 / 982

页数：4

共 21 条

[1] CONJUGATIVE TRANSFER BY THE VIRULENCE SYSTEM OF AGROBACTERIUM-TUMEFACIENS
BEIJERSBERGEN, A
DENDULKRAS, A
SCHILPEROORT, RA
HOOYKAAS, PJJ
[J]. SCIENCE, 1992, 256 (5061) : 1324 - 1327
[2] TRANSKINGDOM T-DNA TRANSFER FROM AGROBACTERIUM-TUMEFACIENS TO SACCHAROMYCES-CEREVISIAE
BUNDOCK, P
DENDULKRAS, A
BEIJERSBERGEN, A
HOOYKAAS, PJJ
[J]. EMBO JOURNAL, 1995, 14 (13) : 3206 - 3214
[3] Bacterial type IV secretion: conjugation systems adapted to deliver effector molecules to host cells
Christie, PJ
Vogel, JP
[J]. TRENDS IN MICROBIOLOGY, 2000, 8 (08) : 354 - 360
[4] NUCLEAR-LOCALIZATION OF AGROBACTERIUM VIRE2 PROTEIN IN PLANT-CELLS
CITOVSKY, V
ZUPAN, J
WARNICK, D
ZAMBRYSKI, P
[J]. SCIENCE, 1992, 256 (5065) : 1802 - 1805
[5] Helicobacter pylori virulence and genetic geography
Covacci, A
Telford, JL
Del Giudice, G
Parsonnet, J
Rappuoli, R
[J]. SCIENCE, 1999, 284 (5418) : 1328 - 1333
[6] Agrobacterium tumefaciens-mediated transformation of filamentous fungi
de Groot, MJA
Bundock, P
Hooykaas, PJJ
Beijersbergen, AGM
[J]. NATURE BIOTECHNOLOGY, 1998, 16 (09) : 839 - 842
[7] Agrobacterium and plant genes involved in T-DNA transfer and integration
Gelvin, SB
[J]. ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 2000, 51 : 223 - 256
[8] Hansen G, 1999, CURR TOP MICROBIOL, V240, P21
[9] Hooykaas PJJ, 2000, BIOLOGY OF PLANT-MICROBE INTERACTIONS, VOL 2, P159
[10] THE VIRULENCE SYSTEM OF AGROBACTERIUM-TUMEFACIENS
HOOYKAAS, PJJ
BEIJERSBERGEN, AGM
[J]. ANNUAL REVIEW OF PHYTOPATHOLOGY, 1994, 32 : 157 - 179

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