Ca2+-dependent activation of guard cell anion channels, triggered by hyperpolarization, is promoted by prolonged depolarization

被引:32
作者
Stange, Annette [1 ]
Hedrich, Rainer [1 ]
Roelfsema, M. Rob G. [1 ]
机构
[1] Univ Wurzburg, Julius von Sachs Inst Biosci, Bioctr, D-97082 Wurzburg, Germany
关键词
S-type anion channel; Ca2+signals; guard cell; Nicotiana tabacum; plasma membrane; voltage-dependent; S-TYPE ANION; CYTOSOLIC-FREE CALCIUM; ABSCISIC-ACID; PLASMA-MEMBRANE; STOMATAL CLOSURE; PROTEIN-KINASES; INTACT PLANTS; SIGNAL-TRANSDUCTION; WILD-TYPE; CA2+;
D O I
10.1111/j.1365-313X.2010.04141.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
P>Rapid stomatal closure is driven by the activation of S-type anion channels in the plasma membrane of guard cells. This response has been linked to Ca2+ signalling, but the impact of transient Ca2+ signals on S-type anion channel activity remains unknown. In this study, transient elevation of the cytosolic Ca2+ level was provoked by voltage steps in guard cells of intact Nicotiana tabacum plants. Changes in the activity of S-type anion channels were monitored using intracellular triple-barrelled micro-electrodes. In cells kept at a holding potential of -100 mV, voltage steps to -180 mV triggered elevation of the cytosolic free Ca2+ concentration. The increase in the cytosolic Ca2+ level was accompanied by activation of S-type anion channels. Guard cell anion channels were activated by Ca2+ with a half maximum concentration of 515 nm (SE = 235) and a mean saturation value of -349 pA (SE = 107) at -100 mV. Ca2+ signals could also be evoked by prolonged (100 sec) depolarization of the plasma membrane to 0 mV. Upon returning to -100 mV, a transient increase in the cytosolic Ca2+ level was observed, activating S-type channels without measurable delay. These data show that cytosolic Ca2+ elevation can activate S-type anion channels in intact guard cells through a fast signalling pathway. Furthermore, prolonged depolarization to 0 mV alters the activity of Ca2+ transport proteins, resulting in an overshoot of the cytosolic Ca2+ level after returning the membrane potential to -100 mV.
引用
收藏
页码:265 / 276
页数:12
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