Modification of capillary electrophoresis selectivity in hydro-organic solutions - Dissociation constants and Stokes radius measurements of peptides in water-2,2,2-trifluoroethanol mixtures
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作者:
Castagnola, M
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机构:Univ Cagliari, Ist Chim Biol, I-09126 Cagliari, Italy
Castagnola, M
Rossetti, DV
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机构:Univ Cagliari, Ist Chim Biol, I-09126 Cagliari, Italy
Rossetti, DV
Misiti, F
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机构:Univ Cagliari, Ist Chim Biol, I-09126 Cagliari, Italy
Misiti, F
Cassiano, L
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机构:Univ Cagliari, Ist Chim Biol, I-09126 Cagliari, Italy
Cassiano, L
Giardina, B
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机构:Univ Cagliari, Ist Chim Biol, I-09126 Cagliari, Italy
Giardina, B
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Messana, I
机构:
[1] Univ Cagliari, Ist Chim Biol, I-09126 Cagliari, Italy
Peptide electrophoretic mobilities were measured at different acidic pH in water-2,2,2-trifluoroethanol mixtures. Fit of experimental mobilities according to binding equations allowed the calculation of peptide C-terminus dissociation constants and the Stokes radius of the differently protonated forms. Analysis of the data showed that the use of hydro-organic solvents in capillary electrophoresis offers the following principal advantages: (a) a modification of dissociation constants and Stokes radii that are strongly dependent upon peptide sequence and that can be conveniently utilised for selectivity manipulation; (b) an increase of separation performance arising from the stabilisation of particular peptide conformations; (c) a solubility of large apolar peptides with respect to aqueous solutions. (C) 1997 Published by Elsevier Science B.V.