A semi-quantitative ELISA for detection of Trypanosoma brucei gambiense specific antibodies in serum and cerebrospinal fluid of sleeping sickness patients

被引：32

作者：

Lejon, V ^{[1
]}

Büscher, P ^{[1
]}

Magnus, E ^{[1
]}

Moons, A ^{[1
]}

Wouters, I ^{[1
]}

Van Meirvenne, N ^{[1
]}

机构：

[1] Inst Trop Med, Dept Parasitol, B-4000 Antwerpen, Belgium

来源：

ACTA TROPICA | 1998年 / 69卷 / 02期

关键词：

sleeping sickness; Trypanosoma brucei gambiense; diagnosis; stage determination; ELISA; IgG; IgM; IgA; albumin; antibody; serum; cerebrospinal fluid;

D O I：

10.1016/S0001-706X(97)00137-X

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A semi-quantitative ELISA, using variable surface glycoprotein of T.b. gambiense as antigen, was developed for the detection of antibodies of different immunoglobulin isotypes in serum and cerebrospinal fluid of sleeping sickness patients. Using the assay, the antibody profiles of paired serum and cerebrospinal fluid samples of 28 patients have been studied. Total concentrations of various Ig isotypes were determined as well. In serum and cerebrospinal fluid a drastic increase in IgG, basically IgG1, as a ell as in IgM levels was observed. The concentration of IgA remained relatively normal. The antitrypanosomal antibodies detected in serum and cerebrospinal fluid were mainly of the IgG (IgG1 and IgG3) and IgM isotypes. Measurement of immunoglobulin and trypanosome specific antibody concentrations in serum and CSF allows calculation of intrathecal antibody synthesis and is a possible tool for determining the clinical stage of sleeping sickness. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：151 / 164

页数：14

共 29 条

[1] HUMAN AFRICAN TRYPANOSOMIASIS - PRESENCE OF ANTIBODIES TO GALACTOCEREBROSIDES
AMEVIGBE, MDD
JAUBERTEAUMARCHAN, MO
BOUTEILLE, B
DOUA, F
BRETON, JC
NICOLAS, JA
DUMAS, M
[J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1992, 47 (05) : 652 - 662
[2] BALTZ T, 1976, ANN INST PASTEUR IMM, VC127, P761
[3] Buscher P, 1995, 22 M INT SCI COUNC T, P46
[4] DEPLA E, 1992, Annales de la Societe Belge de Medecine Tropicale, V72, P96
[5] SLEEPING SICKNESS
DUMAS, M
GIRARD, PL
[J]. TRENDS IN NEUROSCIENCES, 1979, 2 (10) : 256 - 258
[6] DUMAS M, 1985, PRESSE MED, V14, P253
[7] THE DIAGNOSTIC-SIGNIFICANCE OF ANTIBODY SPECIFICITY INDEXES IN MULTIPLE-SCLEROSIS AND HERPES-VIRUS INDUCED DISEASES OF THE NERVOUS-SYSTEM
FELGENHAUER, K
REIBER, H
[J]. CLINICAL INVESTIGATOR, 1992, 70 (01): : 28 - 37
[8] THE PATHOGENESIS OF SLEEPING SICKNESS
GREENWOOD, BM
WHITTLE, HC
[J]. TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1980, 74 (06) : 716 - 725
[9] Hamilton RG, 1989, HUMAN IGG SUBCLASSES
[10] KNOBLOCH J, 1984, TROPENMED PARASITOL, V35, P137

← 1 2 3 →