Nucleotide excision repair in yeast

被引:288
作者
Prakash, S [1 ]
Prakash, L [1 ]
机构
[1] Univ Texas, Med Branch, Sealy Ctr Mol Sci, Galveston, TX 77555 USA
关键词
nucleotide excision repair; yeast; transcription factor;
D O I
10.1016/S0027-5107(00)00037-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In nucleotide excision repair (NER) in eukaryotes, DNA is incised on both sides of the lesion, resulting in the removal of a fragment similar to 35-30 nucleotides long. This is followed by repair synthesis and ligation. The proteins encoded by the various yeast NER genes have been purified, and the incision reaction reconstituted in vitro. This reaction requires the damage binding factors Rad14, RPA, and the Rad4-Rad23 complex, the transcription factor TFIIH which contains the two DNA helicases Rad3 and Rad25, essential for creating a bubble structure, and the two endonucleases, the Rad1-Rad10 complex and Rad2, which incise the damaged DNA strand on the 5'- and S'-side of the lesion, respectively. Addition of the Rad7-Rad16 complex to this reconstituted system stimulates the incision reaction many fold. The various NER proteins exist in vivo as part of multiprotein subassemblies which have been named NEFs (nucleotide excision repair factors). Rad14 and Rad1-Rad10 form one subassembly called NEF1, the Rad4-Rad23 complex is named NEF?, Rad2 and TFIIH constitute NEF3, and the Rad7-Rad16 complex is called NEF4. Although much has been learned from yeast about the function of NER genes and proteins in eukaryotes, the underlying mechanisms by which damage is recognized, NEFs are assembled at the damage site, and the DNA is unwound and incised, remain to be elucidated. (C) 2000 Elsevier Science B.V. Al rights reserved.
引用
收藏
页码:13 / 24
页数:12
相关论文
共 113 条
[91]   RAD3 PROTEIN OF SACCHAROMYCES-CEREVISIAE IS A DNA HELICASE [J].
SUNG, P ;
PRAKASH, L ;
MATSON, SW ;
PRAKASH, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (24) :8951-8955
[92]   HUMAN XERODERMA-PIGMENTOSUM GROUP-D GENE ENCODES A DNA HELICASE [J].
SUNG, P ;
BAILLY, V ;
WEBER, C ;
THOMPSON, LH ;
PRAKASH, L ;
PRAKASH, S .
NATURE, 1993, 365 (6449) :852-855
[93]   MUTATION OF LYSINE-48 TO ARGININE IN THE YEAST RAD3 PROTEIN ABOLISHES ITS ATPASE AND DNA HELICASE ACTIVITIES BUT NOT THE ABILITY TO BIND ATP [J].
SUNG, P ;
HIGGINS, D ;
PRAKASH, L ;
PRAKASH, S .
EMBO JOURNAL, 1988, 7 (10) :3263-3269
[94]   DIFFERENT FORMS OF TFIIH FOR TRANSCRIPTION AND DNA-REPAIR - HOLO-TFIIH AND A NUCLEOTIDE EXCISION REPAIROSOME [J].
SVEJSTRUP, JQ ;
WANG, ZG ;
FEAVER, WJ ;
WU, XH ;
BUSHNELL, DA ;
DONAHUE, TF ;
FRIEDBERG, EC ;
KORNBERG, RD .
CELL, 1995, 80 (01) :21-28
[95]   ANALYSIS OF A HUMAN DNA EXCISION REPAIR GENE INVOLVED IN GROUP-A XERODERMA-PIGMENTOSUM AND CONTAINING A ZINC-FINGER DOMAIN [J].
TANAKA, K ;
MIURA, N ;
SATOKATA, I ;
MIYAMOTO, I ;
YOSHIDA, MC ;
SATOH, Y ;
KONDO, S ;
YASUI, A ;
OKAYAMA, H ;
OKADA, Y .
NATURE, 1990, 348 (6296) :73-76
[96]  
THOMAS BJ, 1989, GENETICS, V123, P725
[97]   Transitions in the coupling of transcription and nucleotide excision repair within RNA polymerase II-transcribed genes of Saccharomyces cerevisiae [J].
Tijsterman, M ;
Verhage, RA ;
vandePutte, P ;
TasserondeJong, JG ;
Brouwer, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (15) :8027-8032
[98]   YEAST DNA-REPAIR AND RECOMBINATION PROTEINS RAD1 AND RAD10 CONSTITUTE A SINGLE-STRANDED-DNA ENDONUCLEASE [J].
TOMKINSON, AE ;
BARDWELL, AJ ;
BARDWELL, L ;
TAPPE, NJ ;
FRIEDBERG, EC .
NATURE, 1993, 362 (6423) :860-862
[99]   ERCC6, A MEMBER OF A SUBFAMILY OF PUTATIVE HELICASES, IS INVOLVED IN COCKAYNES-SYNDROME AND PREFERENTIAL REPAIR OF ACTIVE GENES [J].
TROELSTRA, C ;
VANGOOL, A ;
DEWIT, J ;
VERMEULEN, W ;
BOOTSMA, D ;
HOEIJMAKERS, JHJ .
CELL, 1992, 71 (06) :939-953
[100]   Sequence-specific and domain-specific DNA repair in xeroderma pigmentosum and Cockayne syndrome cells [J].
Tu, YQ ;
Bates, S ;
Pfeifer, GP .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (33) :20747-20755