Auxiliary-mediated site-specific peptide ubiquitylation

被引：152

作者：

Chatterjee, Champak ^{[1
]}

McGinty, Robert K. ^{[1
]}

Pellois, Jean-Philippe ^{[1
]}

Muir, Tom W. ^{[1
]}

机构：

[1] Rockefeller Univ, Lab Synth Prot Chem, New York, NY 10021 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 16期

关键词：

ligation auxiliaries; peptides; semisynthesis; ubiquitylation;

D O I：

10.1002/anie.200605155

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Lighting up ubiquitylation: A photolytically removable ligation auxiliary (Aux) was employed for the site-specific attachment of ubiquitin (Ub) to one of three lysine residues in a C-terminal peptide fragment of histone H2B (cH2B). Following photolysis, the ligation product was a viable substrate for the ubiquitin C-terminal hydrolase UCH-L3. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2814 / 2818

页数：5