Applications of LC/MS in forensic chemistry

被引:21
作者
Nishikawa, M [1 ]
Tsuchihashi, H [1 ]
机构
[1] Osaka Prefectural Police HQ, Forens Sci Lab, Chuo Ku, Osaka 541, Japan
来源
JOURNAL OF TOXICOLOGY-TOXIN REVIEWS | 1998年 / 17卷 / 01期
关键词
D O I
10.3109/15569549809006487
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Various analytical techniques for highly sensitive liquid chromatography/mass spectrometry (LC/MS) and their applications in forensic chemistry have been investigated. The following three types of LC/MS instruments were used for this study: 1) a Micromass model PLATFORM II equipped with an atmospheric pressure chemical ionization (APCI) or an electrospray ionization (ESI) interface; 2) a Shimadzu model QP-1100EX combined with a thermospray TSP interface; 3) a JEOL model JMS-SX102A fitted with a Frit - fast atom bombardment ionization (Frit-FAB) interface. Optimization of capillary voltage and the flow rate of the mobile phase were found for ESI-LC/MS. The relationship between the composition of mobile phase and ionization efficiency was investigated in the ESI, APCI and TSP types of LC/MS. In ESI-LC/MS analysis, the use of a semi-micro column (1.5 mm I.D.) allowed about ten rimes more sensitive an analysis than a conventional column (4.6 mm I.D.). In order to examine the provable period of triazolam, which is quickly metabolized, urine samples obtained from four volunteers who had taken 0.25 or 0.5 mg of the drug were analyzed by TSP-LC,IRIS. The main urinary metabolite of triazolam, alpha-hydroxytriazolam (alpha-HT) was detectable up to 36 hours after taking the dosage. For the purpose of checking the detectable period of suxamethonium in post-mortem biological samples, Frit-FAB LC/MS analysis was performed for the tissue samples of the rats which died by the intraperitoneal administration of the drug at 10 mg/kg bodyweight. Suxamethonium in the rat liver was detectable up to 42 days later, as long as the bodies were stood at 0 degrees C immediately after the death.
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页码:13 / 26
页数:14
相关论文
共 10 条
  • [1] Direct high-performance liquid chromatographic and highperformance liquid chromatographic thermospray-mass spectrometric determination of enantiomers of methamphetamine and its main metabolites amphetamine and p-hydroxymethamphetamine in human urine
    Katagi, M
    Nishioka, H
    Nakajima, K
    Tsuchihashi, H
    Fujima, H
    Wada, H
    Nakamura, K
    Makino, K
    [J]. JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1996, 676 (01): : 35 - 43
  • [2] KATAGI M, 1994, JPN J TOX ENV HEALTH, V40, P357
  • [3] NISHIKAWA M, 1992, JPN J TOX ENV HEALTH, V38, P121
  • [4] THE ANALYSIS OF COCAINE AND ITS METABOLITES BY LIQUID-CHROMATOGRAPHY ATMOSPHERIC-PRESSURE CHEMICAL-IONIZATION MASS-SPECTROMETRY (LC/APCI-MS)
    NISHIKAWA, M
    NAKAJIMA, K
    TATSUNO, M
    KASUYA, F
    IGARASHI, K
    FUKUI, M
    TSUCHIHASHI, H
    [J]. FORENSIC SCIENCE INTERNATIONAL, 1994, 66 (03) : 149 - 158
  • [5] NISHIKAWA M, 1994, JPN J TOX ENV HEALTH, V40, P534
  • [6] NISHIKAWA M, 1995, JPN J TOX ENV HEALTH, V41, P59
  • [7] Simultaneous determination of illicit drugs in human urine by liquid chromatography mass spectrometry
    Tatsuno, M
    Nishikawa, M
    Katagi, M
    Tsuchihashi, H
    [J]. JOURNAL OF ANALYTICAL TOXICOLOGY, 1996, 20 (05) : 281 - 286
  • [8] TATSUNO M, 1992, JPN J TOX ENV HEALTH, V38, P162
  • [9] Tatsuno M, 1996, JPN J TOX ENV HEALTH, V42, P248
  • [10] Tsuchihashi H, 1997, JPN J TOX ENV HEALTH, V43, P108