Antiangiogenic properties of fibstatin, an extracellular FGF-2-binding polypeptide

被引:30
作者
Bossard, C
Van den Berghe, L
Laurell, H
Castano, C
Cerutti, M
Prats, AC
Prats, H
机构
[1] CHU Rangueil, INSERM U589, F-31403 Toulouse 04, France
[2] CNRS, INRA, UMR St Christol, Les Ales, France
关键词
D O I
10.1158/0008-5472.CAN-04-0287
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
By using the two-hybrid system with basic fibroblast growth factor (FGF-2) as bait, we isolated and characterized fibstatin, an endogenous M-r 29,000 human basement membrane-derived inhibitor of angiogenesis and tumor growth. Fibstatin, a fragment containing the type III domains 12-14 of fibronectin, was produced as a recombinant protein and was shown to inhibit the proliferation, migration, and differentiation of endothelial cells in vitro. Antiangiogenic activity of fibstatin was confirmed in a Matrigel angiogenesis assay in vivo, and electrotransfer of the fibstatin gene into muscle tissue resulted in reduced B16F10 tumor growth. Taken together, these results suggest that fibstatin could act as a powerful molecule for antiangiogenic therapy.
引用
收藏
页码:7507 / 7512
页数:6
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