Genotyping of 44 isolates of Fusarium solani, the main agent of fungal keratitis in Brazil

被引:50
作者
Godoy, P
Cano, J
Gené, J
Guarro, J
Höfling-Lima, AL
Colombo, AL
机构
[1] Univ Rovira & Virgili, Fac Med & Ciencies Salut, Dept Ciencies Med Bas, Unitat Microbiol, Tarragona 43201, Spain
[2] Univ Fed Sao Paulo, DIPA, Sao Paulo, Brazil
[3] Univ Fed Sao Paulo, Dept Oftalmol, Sao Paulo, Brazil
关键词
D O I
10.1128/JCM.42.10.4494-4497.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In a retrospective study performed over 6 years in Brazil, Fusarium solani was found to be the most common species causing mycotic keratitis. The genetic diversity of 44 isolates from 39 patients was assessed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and PCR restriction fragment length polymorphism (PCR-RFLP) fingerprinting. ERIC-PCR was more discriminatory than PCR-RFLP for differentiating the strains. By combining of the results of both techniques, we identified 40 genotypes. Molecular typing revealed a high genomic heterogeneity of the strains of F. solani studied.
引用
收藏
页码:4494 / 4497
页数:4
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