共 48 条
Caspase-8 can be activated by interchain proteolysis without receptor-triggered dimerization during drug-induced apoptosis
被引:88
作者:

Sohn, D
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Dusseldorf, Inst Mol Med, D-40225 Dusseldorf, Germany Univ Dusseldorf, Inst Mol Med, D-40225 Dusseldorf, Germany

Schulze-Osthoff, K
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Dusseldorf, Inst Mol Med, D-40225 Dusseldorf, Germany Univ Dusseldorf, Inst Mol Med, D-40225 Dusseldorf, Germany

Jänicke, RU
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Dusseldorf, Inst Mol Med, D-40225 Dusseldorf, Germany Univ Dusseldorf, Inst Mol Med, D-40225 Dusseldorf, Germany
机构:
[1] Univ Dusseldorf, Inst Mol Med, D-40225 Dusseldorf, Germany
关键词:
D O I:
10.1074/jbc.M408585200
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Proteases of the caspase family are thought to be activated by proteolytic processing of their inactive zymogens. However, although proteolytic cleavage is sufficient for executioner caspases, a different mechanism has been recently proposed for initiator caspases, such as caspase-8, which are believed to be activated by proximity-induced dimerization. According to this model, dimerization rather than proteolytic processing is considered as the critical event for caspase-8 activation. Such a mechanism would suggest that in the absence of a dimerization platform such as the death-inducing signaling complex, caspase-8 proteolytic cleavage would result in an inactive enzyme. As several studies have described caspase-8 cleavage during mitochondrial apoptosis, we now investigated whether caspase-8 becomes indeed catalytically active in this pathway. Using an in vivo affinity labeling approach, we demonstrate that caspase-8 is activated in etoposide-treated cells in vivo in the absence of the receptor-induced death-inducing signaling complex formation. Furthermore, we show that both caspase-3 and -6 are required for the efficient activation of caspase-8. Our data therefore indicate that interchain cleavage of caspase-8 in the mitochondrial pathway is sufficient to produce an active enzyme even in the absence of receptor-driven procaspase-8 dimerization.
引用
收藏
页码:5267 / 5273
页数:7
相关论文
共 48 条
[11]
Insights into the regulatory mechanism for caspase-8 activation
[J].
Donepudi, M
;
Mac Sweeney, A
;
Briand, C
;
Grütter, MG
.
MOLECULAR CELL,
2003, 11 (02)
:543-549

Donepudi, M
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Zurich, Inst Biochem, CH-8057 Zurich, Switzerland Univ Zurich, Inst Biochem, CH-8057 Zurich, Switzerland

论文数: 引用数:
h-index:
机构:

Briand, C
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Zurich, Inst Biochem, CH-8057 Zurich, Switzerland Univ Zurich, Inst Biochem, CH-8057 Zurich, Switzerland

Grütter, MG
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Zurich, Inst Biochem, CH-8057 Zurich, Switzerland Univ Zurich, Inst Biochem, CH-8057 Zurich, Switzerland
[12]
Mammalian caspases: Structure, activation, substrates, and functions during apoptosis
[J].
Earnshaw, WC
;
Martins, LM
;
Kaufmann, SH
.
ANNUAL REVIEW OF BIOCHEMISTRY,
1999, 68
:383-424

Earnshaw, WC
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Edinburgh, Inst Cell & Mol Biol, Edinburgh EH9 3JR, Midlothian, Scotland Univ Edinburgh, Inst Cell & Mol Biol, Edinburgh EH9 3JR, Midlothian, Scotland

Martins, LM
论文数: 0 引用数: 0
h-index: 0
机构: Univ Edinburgh, Inst Cell & Mol Biol, Edinburgh EH9 3JR, Midlothian, Scotland

Kaufmann, SH
论文数: 0 引用数: 0
h-index: 0
机构: Univ Edinburgh, Inst Cell & Mol Biol, Edinburgh EH9 3JR, Midlothian, Scotland
[13]
Caspase-8/FLICE functions as an executioner caspase in anticancer drug-induced apoptosis
[J].
Engels, IH
;
Stepczynska, A
;
Stroh, C
;
Lauber, K
;
Berg, C
;
Schwenzer, R
;
Wajant, H
;
Jänicke, RUU
;
Porter, AG
;
Belka, C
;
Gregor, M
;
Schulze-Osthoff, K
;
Wesselborg, S
.
ONCOGENE,
2000, 19 (40)
:4563-4573

Engels, IH
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Stepczynska, A
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Stroh, C
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Lauber, K
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Berg, C
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Schwenzer, R
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

论文数: 引用数:
h-index:
机构:

Jänicke, RUU
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Porter, AG
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Belka, C
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Gregor, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Schulze-Osthoff, K
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Wesselborg, S
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany
[14]
Post-translational modification of bid has differential effects on its susceptibility to cleavage by caspase 8 or caspase 3
[J].
Esposti, MD
;
Ferry, G
;
Masdehors, P
;
Boutin, JA
;
Hickman, JA
;
Dive, C
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2003, 278 (18)
:15749-15757

Esposti, MD
论文数: 0 引用数: 0
h-index: 0
机构: Univ Manchester, Sch Biol Sci, Canc Res UK Cellular & Mol Pharmacol Grp, Manchester M13 9PT, Lancs, England

Ferry, G
论文数: 0 引用数: 0
h-index: 0
机构: Univ Manchester, Sch Biol Sci, Canc Res UK Cellular & Mol Pharmacol Grp, Manchester M13 9PT, Lancs, England

Masdehors, P
论文数: 0 引用数: 0
h-index: 0
机构: Univ Manchester, Sch Biol Sci, Canc Res UK Cellular & Mol Pharmacol Grp, Manchester M13 9PT, Lancs, England

Boutin, JA
论文数: 0 引用数: 0
h-index: 0
机构: Univ Manchester, Sch Biol Sci, Canc Res UK Cellular & Mol Pharmacol Grp, Manchester M13 9PT, Lancs, England

Hickman, JA
论文数: 0 引用数: 0
h-index: 0
机构: Univ Manchester, Sch Biol Sci, Canc Res UK Cellular & Mol Pharmacol Grp, Manchester M13 9PT, Lancs, England

Dive, C
论文数: 0 引用数: 0
h-index: 0
机构: Univ Manchester, Sch Biol Sci, Canc Res UK Cellular & Mol Pharmacol Grp, Manchester M13 9PT, Lancs, England
[15]
Multiple species of CPP32 and Mch2 are the major active caspases present in apoptotic cells
[J].
Faleiro, L
;
Kobayashi, R
;
Fearnhead, H
;
Lazebnik, Y
.
EMBO JOURNAL,
1997, 16 (09)
:2271-2281

Faleiro, L
论文数: 0 引用数: 0
h-index: 0
机构:
SUNY STONY BROOK,GRAD PROGRAM MOL & CELLULAR BIOL,STONY BROOK,NY 11794 SUNY STONY BROOK,GRAD PROGRAM MOL & CELLULAR BIOL,STONY BROOK,NY 11794

Kobayashi, R
论文数: 0 引用数: 0
h-index: 0
机构:
SUNY STONY BROOK,GRAD PROGRAM MOL & CELLULAR BIOL,STONY BROOK,NY 11794 SUNY STONY BROOK,GRAD PROGRAM MOL & CELLULAR BIOL,STONY BROOK,NY 11794

Fearnhead, H
论文数: 0 引用数: 0
h-index: 0
机构:
SUNY STONY BROOK,GRAD PROGRAM MOL & CELLULAR BIOL,STONY BROOK,NY 11794 SUNY STONY BROOK,GRAD PROGRAM MOL & CELLULAR BIOL,STONY BROOK,NY 11794

Lazebnik, Y
论文数: 0 引用数: 0
h-index: 0
机构:
SUNY STONY BROOK,GRAD PROGRAM MOL & CELLULAR BIOL,STONY BROOK,NY 11794 SUNY STONY BROOK,GRAD PROGRAM MOL & CELLULAR BIOL,STONY BROOK,NY 11794
[16]
In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains
[J].
FernandesAlnemri, T
;
Armstrong, RC
;
Krebs, J
;
Srinivasula, SM
;
Wang, L
;
Bullrich, F
;
Fritz, LC
;
Trapani, JA
;
Tomaselli, KJ
;
Litwack, G
;
Alnemri, ES
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
1996, 93 (15)
:7464-7469

FernandesAlnemri, T
论文数: 0 引用数: 0
h-index: 0
机构: THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT PHARMACOL,CTR APOPTOSIS RES,PHILADELPHIA,PA 19107

Armstrong, RC
论文数: 0 引用数: 0
h-index: 0
机构: THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT PHARMACOL,CTR APOPTOSIS RES,PHILADELPHIA,PA 19107

Krebs, J
论文数: 0 引用数: 0
h-index: 0
机构: THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT PHARMACOL,CTR APOPTOSIS RES,PHILADELPHIA,PA 19107

Srinivasula, SM
论文数: 0 引用数: 0
h-index: 0
机构: THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT PHARMACOL,CTR APOPTOSIS RES,PHILADELPHIA,PA 19107

Wang, L
论文数: 0 引用数: 0
h-index: 0
机构: THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT PHARMACOL,CTR APOPTOSIS RES,PHILADELPHIA,PA 19107

Bullrich, F
论文数: 0 引用数: 0
h-index: 0
机构: THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT PHARMACOL,CTR APOPTOSIS RES,PHILADELPHIA,PA 19107

Fritz, LC
论文数: 0 引用数: 0
h-index: 0
机构: THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT PHARMACOL,CTR APOPTOSIS RES,PHILADELPHIA,PA 19107

Trapani, JA
论文数: 0 引用数: 0
h-index: 0
机构: THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT PHARMACOL,CTR APOPTOSIS RES,PHILADELPHIA,PA 19107

Tomaselli, KJ
论文数: 0 引用数: 0
h-index: 0
机构: THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT PHARMACOL,CTR APOPTOSIS RES,PHILADELPHIA,PA 19107

Litwack, G
论文数: 0 引用数: 0
h-index: 0
机构: THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT PHARMACOL,CTR APOPTOSIS RES,PHILADELPHIA,PA 19107

Alnemri, ES
论文数: 0 引用数: 0
h-index: 0
机构: THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT PHARMACOL,CTR APOPTOSIS RES,PHILADELPHIA,PA 19107
[17]
Differential regulation and ATP requirement for caspase-8 and caspase-3 activation during CD95- and anticancer drug-induced apoptosis
[J].
Ferrari, D
;
Stepczynska, A
;
Los, M
;
Wesselborg, S
;
Schulze-Osthoff, K
.
JOURNAL OF EXPERIMENTAL MEDICINE,
1998, 188 (05)
:979-984

Ferrari, D
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Stepczynska, A
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Los, M
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Wesselborg, S
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Schulze-Osthoff, K
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany
[18]
Caspase 8-mediated cleavage of the pro-apoptotic BCL-2 family member BID in p53-dependent apoptosis
[J].
Fischer, B
;
Coelho, D
;
Dufour, P
;
Bergerat, JP
;
Denis, JM
;
Gueulette, J
;
Bischoff, P
.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,
2003, 306 (02)
:516-522

Fischer, B
论文数: 0 引用数: 0
h-index: 0
机构: Univ Strasbourg, Lab Cancerol Expt & Radiobiol, EA 3430, Inst Rech Contre Canc Appareil Digestif, Strasbourg, France

Coelho, D
论文数: 0 引用数: 0
h-index: 0
机构: Univ Strasbourg, Lab Cancerol Expt & Radiobiol, EA 3430, Inst Rech Contre Canc Appareil Digestif, Strasbourg, France

Dufour, P
论文数: 0 引用数: 0
h-index: 0
机构: Univ Strasbourg, Lab Cancerol Expt & Radiobiol, EA 3430, Inst Rech Contre Canc Appareil Digestif, Strasbourg, France

Bergerat, JP
论文数: 0 引用数: 0
h-index: 0
机构: Univ Strasbourg, Lab Cancerol Expt & Radiobiol, EA 3430, Inst Rech Contre Canc Appareil Digestif, Strasbourg, France

Denis, JM
论文数: 0 引用数: 0
h-index: 0
机构: Univ Strasbourg, Lab Cancerol Expt & Radiobiol, EA 3430, Inst Rech Contre Canc Appareil Digestif, Strasbourg, France

Gueulette, J
论文数: 0 引用数: 0
h-index: 0
机构: Univ Strasbourg, Lab Cancerol Expt & Radiobiol, EA 3430, Inst Rech Contre Canc Appareil Digestif, Strasbourg, France

Bischoff, P
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Strasbourg, Lab Cancerol Expt & Radiobiol, EA 3430, Inst Rech Contre Canc Appareil Digestif, Strasbourg, France Univ Strasbourg, Lab Cancerol Expt & Radiobiol, EA 3430, Inst Rech Contre Canc Appareil Digestif, Strasbourg, France
[19]
Many cuts to ruin:: a comprehensive update of caspase substrates
[J].
Fischer, U
;
Jänicke, RU
;
Schulze-Osthoff, K
.
CELL DEATH AND DIFFERENTIATION,
2003, 10 (01)
:76-100

Fischer, U
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Dusseldorf, Inst Mol Med, D-40225 Dusseldorf, Germany Univ Dusseldorf, Inst Mol Med, D-40225 Dusseldorf, Germany

Jänicke, RU
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Dusseldorf, Inst Mol Med, D-40225 Dusseldorf, Germany Univ Dusseldorf, Inst Mol Med, D-40225 Dusseldorf, Germany

Schulze-Osthoff, K
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Dusseldorf, Inst Mol Med, D-40225 Dusseldorf, Germany Univ Dusseldorf, Inst Mol Med, D-40225 Dusseldorf, Germany
[20]
Inhibition of human caspases by peptide-based and macromolecular inhibitors
[J].
Garcia-Calvo, M
;
Peterson, EP
;
Leiting, B
;
Ruel, R
;
Nicholson, DW
;
Thornberry, NA
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
1998, 273 (49)
:32608-32613

Garcia-Calvo, M
论文数: 0 引用数: 0
h-index: 0
机构: Merck Res Labs, Dept Enzymol, Rahway, NJ 07065 USA

Peterson, EP
论文数: 0 引用数: 0
h-index: 0
机构: Merck Res Labs, Dept Enzymol, Rahway, NJ 07065 USA

Leiting, B
论文数: 0 引用数: 0
h-index: 0
机构: Merck Res Labs, Dept Enzymol, Rahway, NJ 07065 USA

Ruel, R
论文数: 0 引用数: 0
h-index: 0
机构: Merck Res Labs, Dept Enzymol, Rahway, NJ 07065 USA

Nicholson, DW
论文数: 0 引用数: 0
h-index: 0
机构: Merck Res Labs, Dept Enzymol, Rahway, NJ 07065 USA

Thornberry, NA
论文数: 0 引用数: 0
h-index: 0
机构: Merck Res Labs, Dept Enzymol, Rahway, NJ 07065 USA