Manipulation and molecular resolution of a phosphatidylcholine-supported planar bilayer by atomic force microscopy

被引:37
作者
Beckmann, M
Nollert, P
Kolb, HA
机构
[1] Univ Hannover, Inst Biophys, D-30419 Hannover, Germany
[2] Becton Dickinson Immunocytometry Syst, D-69126 Heidelberg, Germany
[3] Univ Basel, Biozentrum, Dept Microbiol, CH-4056 Basel, Switzerland
关键词
lipid head groups; packing order;
D O I
10.1007/s002329900329
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The morphology of supported planar bilayers has been investigated below phase transition temperature by atomic force microscopy in contact and tapping mode. The bilayers were formed by the vesicle-spreading technique. In contact mode at low scanning forces of about 1 nN true molecular resolution could be achieved for supported phosphatidylcholine bilayers. The resolution was confirmed by experiments that captured the location, average area of individual lipid headgroups and the manipulation of the bilayer surface. Repeated scanning in contact mode shifted the random topology of the surface consecutively to a striped pattern. Height profiles of defect-containing bilayers were analyzed. The shape of the defects became smooth by repeated scanning. The height profiles allowed the estimation of the indentation of the tip into the surface-adsorbed membrane. In tapping mode a disordered pattern of headgroups became visible. Our morphological data at molecular resolution suggest that the native arrangement of the choline head-groups is disordered, free of large packing defects and becomes ordered in Schallamach waves by scanning in contact mode.
引用
收藏
页码:227 / 233
页数:7
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