Effect of bacterial inoculation, plant genotype and developmental stage on root-associated and endophytic bacterial communities in potato (Solanum tuberosum)

被引:83
作者
Andreote, Fernando Dini [1 ,2 ]
da Rocha, Ulisses Nunes [2 ]
Araujo, Welington Luiz [3 ]
Azevedo, Joao Lucio [4 ]
van Overbeek, Leonard Simon [2 ]
机构
[1] USP, ESALQ, Dept Ciencia Solo, BR-13418900 Piracicaba, SP, Brazil
[2] Plant Res Int, Wageningen, Netherlands
[3] Univ Mogi das Cruzes, Lab Microbial Ecol & Mol Biol, Mogi Das Cruzes, SP, Brazil
[4] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, Piracicaba, SP, Brazil
来源
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2010年 / 97卷 / 04期
关键词
PCR-DGGE; Methylobacterium; Paenibacillus; Multivariate analysis; Bacterial inoculation; GRADIENT GEL-ELECTROPHORESIS; 16S RIBOSOMAL-RNA; LAND-USE HISTORY; TRANSGENIC EUCALYPTUS; MULTIVARIATE ANALYSES; XYLELLA-FASTIDIOSA; WILD-TYPE; RHIZOSPHERE; DIVERSITY; SOIL;
D O I
10.1007/s10482-010-9421-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Beneficial bacteria interact with plants by colonizing the rhizosphere and roots followed by further spread through the inner tissues, resulting in endophytic colonization. The major factors contributing to these interactions are not always well understood for most bacterial and plant species. It is believed that specific bacterial functions are required for plant colonization, but also from the plant side specific features are needed, such as plant genotype (cultivar) and developmental stage. Via multivariate analysis we present a quantification of the roles of these components on the composition of root-associated and endophytic bacterial communities in potato plants, by weighing the effects of bacterial inoculation, plant genotype and developmental stage. Spontaneous rifampicin resistant mutants of two bacterial endophytes, Paenibacillus sp. strain E119 and Methylobacterium mesophilicum strain SR1.6/6, were introduced into potato plants of three different cultivars (Eersteling, Robijn and Karnico). Densities of both strains in, or attached to potato plants were measured by selective plating, while the effects of bacterial inoculation, plant genotype and developmental stage on the composition of bacterial, Alphaproteobacterial and Paenibacillus species were determined by PCR-denaturing gradient gel-electrophoresis (DGGE). Multivariate analyses revealed that the composition of bacterial communities was mainly driven by cultivar type and plant developmental stage, while Alphaproteobacterial and Paenibacillus communities were mainly influenced by bacterial inoculation. These results are important for better understanding the effects of bacterial inoculations to plants and their possible effects on the indigenous bacterial communities in relation with other plant factors such as genotype and growth stage.
引用
收藏
页码:389 / 399
页数:11
相关论文
共 42 条
[31]   PROFILING OF COMPLEX MICROBIAL-POPULATIONS BY DENATURING GRADIENT GEL-ELECTROPHORESIS ANALYSIS OF POLYMERASE CHAIN REACTION-AMPLIFIED GENES-CODING FOR 16S RIBOSOMAL-RNA [J].
MUYZER, G ;
DEWAAL, EC ;
UITTERLINDEN, AG .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (03) :695-700
[32]   Plant colonization by pink-pigmented facultative methylotrophic bacteria (PPFMs) [J].
Omer, ZS ;
Tombolini, R ;
Gerhardson, B .
FEMS MICROBIOLOGY ECOLOGY, 2004, 47 (03) :319-326
[33]  
Oros-Sichler R., 2007, MODERN SOIL MICROBIO, Vsecond, P355
[34]   Multivariate analyses in microbial ecology [J].
Ramette, Alban .
FEMS MICROBIOLOGY ECOLOGY, 2007, 62 (02) :142-160
[35]   Rhizosphere bacteria affected by transgenic potatoes with antibacterial activities compared with the effects of soil, wild-type potatoes, vegetation stage and pathogen exposure [J].
Rasche, F ;
Hödl, V ;
Poll, C ;
Kandeler, E ;
Gerzabek, MH ;
van Elsas, JD ;
Sessitsch, A .
FEMS MICROBIOLOGY ECOLOGY, 2006, 56 (02) :219-235
[36]   Bacterial endophytes and their interactions with hosts [J].
Rosenblueth, Monica ;
Martinez-Romero, Esperanza .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2006, 19 (08) :827-837
[37]   Multivariate analyses of Burkholderia species in soil:: Effect of crop and land use history [J].
Salles, JF ;
van Veen, JA ;
van Elsas, JD .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (07) :4012-4020
[38]   Soil microbial community composition and land use history in cultivated and grassland ecosystems of coastal California [J].
Steenwerth, KL ;
Jackson, LE ;
Calderón, FJ ;
Stromberg, MR ;
Scow, KM .
SOIL BIOLOGY & BIOCHEMISTRY, 2002, 34 (11) :1599-1611
[39]  
ter Braak C.J. F., 1988, CANOCO REFERENCE MAN
[40]   Effect of soil sample preservation, compared to the effect of other environmental variables, on bacterial and eukaryotic diversity [J].
Tzeneva, Vesela A. ;
Salles, Joana F. ;
Naumova, Natalia ;
de Vos, Willem A. ;
Kuikman, Peter J. ;
Dolfing, Jan ;
Smidt, Hauke .
RESEARCH IN MICROBIOLOGY, 2009, 160 (02) :89-98