Interactions and phase transitions in protein solutions

被引：135

作者：

Piazza, R ^{[1
]}

机构：

[1] Politecn Milan, Dipartimento Ingn Nucl, Unita INFM, I-20133 Milan, Italy

来源：

CURRENT OPINION IN COLLOID & INTERFACE SCIENCE | 2000年 / 5卷 / 1-2期

关键词：

proteins; colloidal suspensions; complex fluids; phase transitions; crystallization; light scattering; aggregation;

D O I：

10.1016/S1359-0294(00)00034-0

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Understanding interactions and phase transitions in protein solutions is essential in order to develop systematic protein crystallisation strategies. Recent studies show that proteins near crystallisation behave as particles interacting via a very short-range attractive potential. The microscopic origin of this attractive term, and its strong dependence on the nature of the crystallisation agent, is, however, still obscure. The interplay of crystal nucleation with formation of weakly bonded amorphous aggregates also deserves further analysis. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：38 / 43

页数：6

共 49 条

[21] STATISTICAL-MECHANICAL MODEL OF PROTEIN PRECIPITATION BY NONIONIC POLYMER [J].

MAHADEVAN, H ;

HALL, CK .

AICHE JOURNAL, 1990, 36 (10) :1517-1528

[22] SALT EFFECTS ON HYDROPHOBIC INTERACTIONS IN PRECIPITATION AND CHROMATOGRAPHY OF PROTEINS - INTERPRETATION OF LYOTROPIC SERIES [J].

MELANDER, W ;

HORVATH, C .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1977, 183 (01) :200-215

[23] The role played by hydration forces in the stability of protein-coated particles:: non-classical DLVO behaviour [J].

Molina-Bolívar, JA ;

Galisteo-González, F ;

Hidalgo-Alvarez, R .

COLLOIDS AND SURFACES B-BIOINTERFACES, 1999, 14 (1-4) :3-17

[24] Liquid-liquid phase separation in supersaturated lysozyme solutions and associated precipitate formation/crystallization [J].

Muschol, M ;

Rosenberger, F .

JOURNAL OF CHEMICAL PHYSICS, 1997, 107 (06) :1953-1962

[25] Interactions in undersaturated and supersaturated lysozyme solutions: Static and dynamic light scattering results [J].

Muschol, M ;

Rosenberger, F .

JOURNAL OF CHEMICAL PHYSICS, 1995, 103 (24) :10424-10432

[26] Ion binding and ion specificity: The Hofmeister effect and Onsager and Lifshitz theories [J].

Ninham, BW ;

Yaminsky, V .

LANGMUIR, 1997, 13 (07) :2097-2108

[27] The role of long-range forces in the phase behavior of colloids and proteins [J].

Noro, MG ;

Kern, N ;

Frenkel, D .

EUROPHYSICS LETTERS, 1999, 48 (03) :332-338

[28] Electrical properties of free surface of water and aqueous solutions [J].

Paluch, M .

ADVANCES IN COLLOID AND INTERFACE SCIENCE, 2000, 84 (1-3) :27-45

[29] Effect of salts on the micellization, clouding, and solubilization behavior of pluronic F127 solutions [J].

Pandit, N ;

Trygstad, T ;

Croy, S ;

Bohorquez, M ;

Koch, C .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2000, 222 (02) :213-220

[30] Evidence for non-DLVO hydration interactions in solutions of the protein apoferritin [J].

Petsev, DN ;

Vekilov, PG .

PHYSICAL REVIEW LETTERS, 2000, 84 (06) :1339-1342

← 1 2 3 4 5 →