A lily stylar pectin is necessary for pollen tube adhesion to an in vitro stylar matrix

被引：150

作者：

Mollet, JC ^{[1
]}

Park, SY ^{[1
]}

Nothnagel, EA ^{[1
]}

Lord, EM ^{[1
]}

机构：

[1] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA

来源：

PLANT CELL | 2000年 / 12卷 / 09期

关键词：

D O I：

10.1105/tpc.12.9.1737

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pollen tube cells adhere to the wall surface of the stylar transmitting tract epidermis in lily. This adhesion has been proposed as essential for the proper delivery of the sperm cells to the ovule. An in vitro adhesion bioassay has been used to isolate two stylar molecules required for lily pollen tube adhesion. The first molecule was determined to be a small, cysteine-rich protein with some sequence similarity to lipid transfer proteins and now called stigma/stylar cysteine-rich adhesin (SCA). The second, larger, molecule has now been purified from style fragments and characterized. Chemical composition, specific enzyme degradations, and immunolabeling data support the idea that this molecule required for pollen tube adhesion is a pectic polysaccharide. In vitro binding assays revealed that this lily stylar adhesive pectin and SCA are able to bind to each other in a pH-dependent manner.

引用

页码：1737 / 1749

页数：13

共 79 条

[1] An hypothesis: The same six polysaccharides are components of the primary cell walls of all higher plants [J].

Albersheim, P ;

Darvill, AG ;

ONeill, MA ;

Schols, HA ;

Voragen, AGJ .

PECTINS AND PECTINASES, 1996, 14 :47-55

[2]

ALLEN AK, 1983, CHEM TAXONOMY MOL BI, P71

[3] A NOVEL HYDROXYPROLINE-DEFICIENT ARABINOGALACTAN PROTEIN SECRETED BY SUSPENSION-CULTURED CELLS OF DAUCUS-CAROTA - PURIFICATION AND PARTIAL CHARACTERIZATION [J].

BALDWIN, TC ;

MCCANN, MC ;

ROBERTS, K .

PLANT PHYSIOLOGY, 1993, 103 (01) :115-123

[4] NEW METHOD FOR QUANTITATIVE-DETERMINATION OF URONIC ACIDS [J].

BLUMENKR.N ;

ASBOEHAN.G .

ANALYTICAL BIOCHEMISTRY, 1973, 54 (02) :484-489

[5] Pectic epitopes are differentially distributed in the cell walls of potato (Solanum tuberosum) tubers [J].

Bush, MS ;

McCann, MC .

PHYSIOLOGIA PLANTARUM, 1999, 107 (02) :201-213

[6]

Carpita N, 1996, PLANT CELL, V8, P1451

[7] STRUCTURAL MODELS OF PRIMARY-CELL WALLS IN FLOWERING PLANTS - CONSISTENCY OF MOLECULAR-STRUCTURE WITH THE PHYSICAL-PROPERTIES OF THE WALLS DURING GROWTH [J].

CARPITA, NC ;

GIBEAUT, DM .

PLANT JOURNAL, 1993, 3 (01) :1-30

[8] Plant cell wall proteins [J].

Cassab, GI .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1998, 49 :281-309

[9] A FLORAL TRANSMITTING TISSUE-SPECIFIC GLYCOPROTEIN ATTRACTS POLLEN TUBES AND STIMULATES THEIR GROWTH [J].

CHEUNG, AY ;

WANG, H ;

WU, HM .

CELL, 1995, 82 (03) :383-393

[10] An adhesin of the yeast pathogen Candida glabrata mediating adherence to human epithelial cells [J].

Cormack, BP ;

Ghori, N ;

Falkow, S .

SCIENCE, 1999, 285 (5427) :578-582

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