A lily stylar pectin is necessary for pollen tube adhesion to an in vitro stylar matrix

被引：150

作者：

Mollet, JC ^{[1
]}

Park, SY ^{[1
]}

Nothnagel, EA ^{[1
]}

Lord, EM ^{[1
]}

机构：

[1] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA

来源：

PLANT CELL | 2000年 / 12卷 / 09期

关键词：

D O I：

10.1105/tpc.12.9.1737

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pollen tube cells adhere to the wall surface of the stylar transmitting tract epidermis in lily. This adhesion has been proposed as essential for the proper delivery of the sperm cells to the ovule. An in vitro adhesion bioassay has been used to isolate two stylar molecules required for lily pollen tube adhesion. The first molecule was determined to be a small, cysteine-rich protein with some sequence similarity to lipid transfer proteins and now called stigma/stylar cysteine-rich adhesin (SCA). The second, larger, molecule has now been purified from style fragments and characterized. Chemical composition, specific enzyme degradations, and immunolabeling data support the idea that this molecule required for pollen tube adhesion is a pectic polysaccharide. In vitro binding assays revealed that this lily stylar adhesive pectin and SCA are able to bind to each other in a pH-dependent manner.

引用

页码：1737 / 1749

页数：13

共 79 条

[41] ACETYL-ESTERIFICATION AND METHYL-ESTERIFICATION OF PECTINS OF FRIABLE AND COMPACT SUGAR-BEET CALLI - CONSEQUENCES FOR INTERCELLULAR-ADHESION [J].

LINERS, F ;

GASPAR, T ;

VANCUTSEM, P .

PLANTA, 1994, 192 (04) :545-556

[42]

Lord E, 1996, MEMBRANES SPECIALIZE, P21

[43] Immunocytochemistry of rhamnogalacturonan II in cell walls of higher plants [J].

Matoh, T ;

Takasaki, M ;

Takabe, K ;

Kobayashi, M .

PLANT AND CELL PHYSIOLOGY, 1998, 39 (05) :483-491

[44]

Mohnen D., 1999, COMPREHENSIVE NATURA, V3, P497

[45] IMMUNOGOLD LOCALIZATION OF XYLOGLUCAN AND RHAMNOGALACTURONAN-I IN THE CELL-WALLS OF SUSPENSION-CULTURED SYCAMORE CELLS [J].

MOORE, PJ ;

DARVILL, AG ;

ALBERSHEIM, P ;

STAEHELIN, LA .

PLANT PHYSIOLOGY, 1986, 82 (03) :787-794

[46] PROBLEMS ENCOUNTERED DURING THE EXTRACTION, PURIFICATION, AND CHROMATOGRAPHY OF PECTIC FRAGMENTS, AND SOME SOLUTIONS TO THEM [J].

MORT, AJ ;

MOERSCHBACHER, BM ;

PIERCE, ML ;

MANESS, NO .

CARBOHYDRATE RESEARCH, 1991, 215 (01) :219-227

[47]

Nothnagel EA, 1997, INT REV CYTOL, V174, P195, DOI 10.1016/S0074-7696(08)62118-X

[48] HOST-PATHOGEN INTERACTIONS .22. A GALACTURONIC ACID OLIGOSACCHARIDE FROM PLANT-CELL WALLS ELICITS PHYTOALEXINS [J].

NOTHNAGEL, EA ;

MCNEIL, M ;

ALBERSHEIM, P ;

DELL, A .

PLANT PHYSIOLOGY, 1983, 71 (04) :916-926

[49]

O'Neill M., 1990, Methods in Plant Biochemistry, P415, DOI [10.1016/B978-0-12-461012-5.50018-5, DOI 10.1016/B978-0-12-461012-5.50018-5]

[50]

Ofek I., 1994, BACTERIAL ADHESION C

← 1 2 3 4 5 6 7 8 →