G1 cyclin-dependent kinases are insufficient to reverse dE2F2-mediated repression

被引：26

作者：

Frolov, MV ^{[1
]}

Stevaux, O ^{[1
]}

Moon, NS ^{[1
]}

Dimova, D ^{[1
]}

Kwon, EJ ^{[1
]}

Morris, EJ ^{[1
]}

Dyson, NJ ^{[1
]}

机构：

[1] Massachusetts Gen Hosp, Ctr Canc, Charlestown, MA 02129 USA

来源：

GENES & DEVELOPMENT | 2003年 / 17卷 / 06期

关键词：

E2F; G1; cyclins; Drosophila;

D O I：

10.1101/gad.1031803

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Here we show that the cell cycle defects of dE2F1-depleted cells depend on the cooperative effects of dE2F2 and DACAPO (DAP), an inhibitor of Cyclin E/cyclindependent kinase 2 (CycE/cdk2). The different properties of cells lacking dE2F1/dE2F2 and dE2F1/DAP lead to the surprising observation that dE2F2-mediated repression differs from retinoblastoma family protein 1 (RBF1) inhibition of dE2F1, and is resistant to both CycE/cdk2 and Cyclin D/cyclin-dependent kinase 4 (CycD/cdk4). This resistance occurs even though dE2F2/RBF1 complexes are disrupted by CycE/cdk2, and may explain why dE2F2 is so potent in the absence of de2f1. The implication of these results is that cells containing dE2F2 require dE2F1 to either prevent, or reverse, dE2F7-mediated repression.

引用

页码：723 / 728

页数：6

共 27 条

[1] Ectopic E2F expression induces S phase and apoptosis in Drosophila imaginal discs [J].

Asano, M ;

Nevins, JR ;

Wharton, RP .

GENES & DEVELOPMENT, 1996, 10 (11) :1422-1432

[2] Retinoblastoma protein meets chromatin [J].

Brehm, A ;

Kouzarides, T .

TRENDS IN BIOCHEMICAL SCIENCES, 1999, 24 (04) :142-145

[3]

Cayirlioglu P, 2001, DEVELOPMENT, V128, P5085

[4] Expression of the cyclin-dependent kinase inhibitor Dacapo is regulated by Cyclin E [J].

de Nooij, JC ;

Graber, KH ;

Hariharan, IK .

MECHANISMS OF DEVELOPMENT, 2000, 97 (1-2) :73-83

[5] A cyclin-dependent kinase inhibitor, dacapo, is necessary for timely exit from the cell cycle during Drosophila embryogenesis [J].

deNooij, JC ;

Letendre, MA ;

Hariharan, IK .

CELL, 1996, 87 (07) :1237-1247

[6] Ectopic expression of dE2F and dDP induces cell proliferation and death in the Drosophila eye [J].

Du, W ;

Xie, JE ;

Dyson, N .

EMBO JOURNAL, 1996, 15 (14) :3684-3692

[7] RBF, a novel RE-related gene that regulates E2F activity and interacts with cyclin E in Drosophila [J].

Du, W ;

Vidal, M ;

Xie, JE ;

Dyson, N .

GENES & DEVELOPMENT, 1996, 10 (10) :1206-1218

[8] THE TRANSCRIPTION FACTOR E2F IS REQUIRED FOR S-PHASE DURING DROSOPHILA EMBRYOGENESIS [J].

DURONIO, RJ ;

OFARRELL, PH ;

XIE, JE ;

BROOK, A ;

DYSON, N .

GENES & DEVELOPMENT, 1995, 9 (12) :1445-1455

[9] DEVELOPMENTAL CONTROL OF THE G(1) TO S-TRANSITION IN DROSOPHILA - CYCLIN-E IS A LIMITING DOWNSTREAM TARGET OF E2F [J].

DURONIO, RJ ;

OFARRELL, PH .

GENES & DEVELOPMENT, 1995, 9 (12) :1456-1468

[10] Functional antagonism between E2F family members [J].

Frolov, MV ;

Huen, DS ;

Stevaux, O ;

Dimova, D ;

Balczarek-Strang, K ;

Elsdon, M ;

Dyson, NJ .

GENES & DEVELOPMENT, 2001, 15 (16) :2146-2160

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