Stability and cellular responses to fluorapatite-collagen composites

被引：69

作者：

Yoon, BH ^{[1
]}

Kim, HW ^{[1
]}

Lee, SH ^{[1
]}

Bae, CJ ^{[1
]}

Koh, YH ^{[1
]}

Kong, YM ^{[1
]}

Kim, HE ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Mat Sci & Engn, Seoul 151744, South Korea

来源：

BIOMATERIALS | 2005年 / 26卷 / 16期

关键词：

fluorapatite (FA); collagen; biomimetic coprecipitation; stability; cellular responses;

D O I：

10.1016/j.biomaterials.2004.07.062

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Fluorapatite (FA)-collagen composites were synthesized via a biomimetic coprecipitation method in order to improve the structural stability and cellular responses. Different amounts of ammonium fluoride (NH4F), acting as a fluorine source for FA, were added to the precipitation of the composites. The precipitated composites were freeze-dried and isostatically pressed in a dense body. The added fluorine was incorporated nearly fully into the apatite structure (fluoridation), and a near stoichiometric FA-collagen composite was obtained with complete fluoridation. The freeze-dried composites had a typical biomimetic network, consisting of collagen fibers and precipitates of nano-sized apatite crystals. The human osteoblast-like cells on the FA-collagen composites exhibited significantly higher proliferation and differentiation (according to alkaline phosphatase activity) than those on the hydroxyapatite-collagen composite. These enhanced osteoblastic cell responses were attributed to the fluorine release and the reduced dissolution rate. (C) 2004 Elsevier Ltd. All fights reserved.

引用

页码：2957 / 2963

页数：7

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