The Southeast Collaboratory for Structural Genomics: A high-throughput gene to structure factory

被引：60

作者：

Adams, MWW

Dailey, HA

Delucas, LJ

Luo, M

Prestegard, JH

Rose, JP

Wang, BC ^{[1
]}

机构：

[1] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA

[2] Univ Alabama Birmingham, Dept Microbiol, Ctr Biophys Sci & Engn, Birmingham, AL 35294 USA

来源：

ACCOUNTS OF CHEMICAL RESEARCH | 2003年 / 36卷 / 03期

关键词：

D O I：

10.1021/ar0101382

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The Southeast Collaboratory for Structural Genomics consists of four working groups. The protein production group supplies/develops high-output production of Pyrococcus furiosus, Caenorhabditis elegans, and selected human proteins. The X-ray crystallography group conducts high-throughput structure production in parallel with production-related research/development in nanocrystallization robotics, capillary crystallization cassette, synchrotron/home X-ray instrumentation, sample mounting robotics, data processing and pipelined structure analysis, combined refinement/validation protocols, and direct use of unlabeled native crystals (Direct Crystallography). The NMR group emphasizes/develops sample screening and backbone structure determination from residual dipolar coupling data. The bioinformatics group implements/develops local database interfaces, pipelined sequence/structure information search/updates, and database/bioinformatics toolkits.

引用

页码：191 / 198

页数：8

共 23 条

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