Structural basis topological specificity function of MinE

被引：65

作者：

King, GF

Shih, YL

Maciejewski, MW

Bains, NPS

Pan, BL

Rowland, SL

Mullen, GP

Rothfield, LI

机构：

[1] Univ Connecticut, Ctr Hlth, Dept Biochem, Farmington, CT 06032 USA

[2] Univ Connecticut, Ctr Hlth, Dept Microbiol, Farmington, CT 06032 USA

来源：

NATURE STRUCTURAL BIOLOGY | 2000年 / 7卷 / 11期

基金：

美国国家卫生研究院; 英国医学研究理事会;

关键词：

D O I：

10.1038/80917

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Correct positioning of the division septum in Escherichia coli depends on the coordinated action of the MinC, MinD and MinE proteins. Topological specificity is conferred on the MinCD division inhibitor by MinE, which counters MinCD activity only in the vicinity of the preferred midcell division site. Here we report the structure of the homodimeric topological specificity domain of Escherichia coli MinE and show that it forms a novel ap sandwich, Structure-directed mutagenesis of conserved surface residues has enabled us to identify a spatially restricted site on the surface of the protein that is critical for the topological specificity function of MinE.

引用

页码：1013 / 1017

页数：5

共 24 条

[1] THE PROGRAM XEASY FOR COMPUTER-SUPPORTED NMR SPECTRAL-ANALYSIS OF BIOLOGICAL MACROMOLECULES
BARTELS, C
XIA, TH
BILLETER, M
GUNTERT, P
WUTHRICH, K
[J]. JOURNAL OF BIOMOLECULAR NMR, 1995, 6 (01) : 1 - 10
[2] BRUNGER AT, 1992, XPLOR VERSION 3 1
[3] 3-DIMENSIONAL SOLUTION STRUCTURE OF ESCHERICHIA-COLI PERIPLASMIC CYCLOPHILIN
CLUBB, RT
FERGUSON, SB
WALSH, CT
WAGNER, G
[J]. BIOCHEMISTRY, 1994, 33 (10) : 2761 - 2772
[4] A DIVISION INHIBITOR AND A TOPOLOGICAL SPECIFICITY FACTOR CODED FOR BY THE MINICELL LOCUS DETERMINE PROPER PLACEMENT OF THE DIVISION SEPTUM IN ESCHERICHIA-COLI
DEBOER, PAJ
CROSSLEY, RE
ROTHFIELD, LI
[J]. CELL, 1989, 56 (04) : 641 - 649
[5] THE MIND PROTEIN IS A MEMBRANE ATPASE REQUIRED FOR THE CORRECT PLACEMENT OF THE ESCHERICHIA-COLI DIVISION SITE
DEBOER, PAJ
CROSSLEY, RE
HAND, AR
ROTHFIELD, LI
[J]. EMBO JOURNAL, 1991, 10 (13) : 4371 - 4380
[6] Torsion angle dynamics for NMR structure calculation with the new program DYANA
Guntert, P
Mumenthaler, C
Wuthrich, K
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1997, 273 (01) : 283 - 298
[7] PROTEIN-STRUCTURE COMPARISON BY ALIGNMENT OF DISTANCE MATRICES
HOLM, L
SANDER, C
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1993, 233 (01) : 123 - 138
[8] Topological regulation of cell division in Escherichia coli involves rapid pole to pole oscillation of the division inhibitor MinC under the control of MinD and MinE
Hu, ZL
Lutkenhaus, J
[J]. MOLECULAR MICROBIOLOGY, 1999, 34 (01) : 82 - 90
[9] Interaction between FtsZ and inhibitors of cell division
Huang, J
Cao, C
Lutkenhaus, J
[J]. JOURNAL OF BACTERIOLOGY, 1996, 178 (17) : 5080 - 5085
[10] Letter to the Editor:: Backbone and side-chain 1H, 15N, and 13C assignments for the topological specificity domain of the MinE cell division protein
King, GF
Pan, BL
Maciejewski, MW
Rowland, SL
Rothfield, LI
Mullen, GP
[J]. JOURNAL OF BIOMOLECULAR NMR, 1999, 13 (04) : 395 - 396

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