Chromosome mosaicism in day 3 aneuploid embryos that develop to morphologically normal blastocysts in vitro

被引:222
作者
Magli, MC
Jones, GM
Gras, L
Gianaroli, L
Korman, I
Trounson, AO
机构
[1] Monash Univ, Ctr Early Human Dev, Monash Inst Reprod & Dev, Clayton, Vic 3168, Australia
[2] SISMER, Reprod Med Unit, I-40138 Bologna, Italy
[3] Monash Private Surg Hosp, Clayton, Vic, Australia
[4] Allamanda Med Ctr, Southport, Qld, Australia
关键词
aneuploidy; blastocyst; chromosomal mosaicism; embryo biopsy; inner cell mass cells;
D O I
10.1093/humrep/15.8.1781
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
In all, 143 human embryos obtained 3 days (day 3) after insemination or intracytoplasmic sperm injection (ICSI) were biopsied and a single nucleated cell removed for identification of aneuploidy by fluorescent in-situ hybridization (FISH) for chromosomes X, Y, 13, 16, 18 and 21, Fifty-one per cent of embryos were aneuploid and significantly more aneuploid embryos blocked in further development to morulae and blastocysts than euploid embryos (59 versus 34%; P < 0.001). Chromosomal analysis of the generated blastocysts revealed 40% were aneuploid (16 of 40 generated blastocysts). Re-examination of cells by FISH for the same chromosome probes of the inner cell mass (ICM) of expanded and hatching blastocysts derived from the aneuploid embryos revealed a high incidence of mosaicism of ICM cell lineages that were usually predictable from observations of day 3 single-cell biopsies, These data would not support the hypothesis of a preferential allocation of euploid cells to the ICM and aneuploid cells to the trophectoderm, A high concordance between day 3 aneuploidy diagnosis and ICM cell lineages was observed with trisomies (97%), and multiple complex chromosome numerical abnormalities (100%), A reduced concordance was observed with monosomies (65%) and haploidy (18%), Concomitantly, the proportion of ICM cell lineages was increased in blastocysts whose chromosomal condition was diagnosed as haploid (21%) or with complex numerical abnormalities (50%).
引用
收藏
页码:1781 / 1786
页数:6
相关论文
共 22 条
[21]   ARE CLINICAL AND BIOLOGICAL IVF PARAMETERS CORRELATED WITH CHROMOSOMAL DISORDERS IN EARLY LIFE - A MULTICENTRIC STUDY [J].
PLACHOT, M ;
VEIGA, A ;
MONTAGUT, J ;
DEGROUCHY, J ;
CALDERON, G ;
LEPRETRE, S ;
JUNCA, AM ;
SANTALO, J ;
CARLES, E ;
MANDELBAUM, J ;
BARRI, P ;
DEGOY, J ;
COHEN, J ;
EGOZCUE, J ;
SABATIER, JC ;
SALATBAROUX, J .
HUMAN REPRODUCTION, 1988, 3 (05) :627-635
[22]  
TRONSON A, 1983, FERTILIZATION HUMAN, P235