Comparative studies on tetrachloroethene reductive dechlorination mediated by Desulfitobacterium sp. strain PCE-S

被引:91
作者
Miller, E [1 ]
Wohlfarth, G [1 ]
Diekert, G [1 ]
机构
[1] Univ Stuttgart, Inst Mikrobiol, D-70569 Stuttgart, Germany
关键词
corrinoids; Dehalospirillum multivorans; Desulfitobacterium; tetrachloroethene dehalogenase; propyl iodide; reductive dechlorination; strain PCE-S; tetrachloroethene; trichloroethene;
D O I
10.1007/s002030050529
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Tetrachloroethene reductive dechlorination was studied with cell extracts of a newly isolated, tetrachloroethene-utilizing bacterium, Desulfitobacterium sp. strain PCE-S. Tetrachloroethene dehalogenase mediated the reductive dechlorination of tetrachloroethene and trichloroethene to cis-1,2-dichloroethene with artificial electron donors such as methyl viologen. The chlorinated aromatic compounds tested so far were not reduced. A low-potential electron donor (E-0' < -0.4 V) was required for tetrachloroethene reduction. The enzyme in its reduced state was inactivated by propyl iodide and reactivated by light, indicating the involvement of a corrinoid in reductive tetrachloroethene dechlorination.
引用
收藏
页码:513 / 519
页数:7
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