EspJ is a prophage-carried type III effector protein of attaching and effacing pathogens that modulates infection dynamics

被引:61
作者
Dahan, S
Wiles, S
La Ragione, RM
Best, A
Woodward, MJ
Stevens, MP
Shaw, RK
Chong, YW
Knutton, S
Phillips, A
Frankel, G
机构
[1] Univ London Imperial Coll Sci Technol & Med, Ctr Mol Microbiol & Infect, Dept Sci Biol, London SW7 2AZ, England
[2] UCL Royal Free & Univ Coll, Sch Med, Ctr Paediat Gastroenterol, London, England
[3] Vet Labs Agcy Defra, Dept Food & Environm Safety, Addlestone, Surrey, England
[4] Inst Anim Hlth, Div Microbiol, Newbury, Berks, England
[5] Univ Birmingham, Inst Child Hlth, Birmingham B15 2TT, W Midlands, England
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1128/IAI.73.2.679-686.2005
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Enterohemorrhagic Escherichia coli, enteropathogenic E. coli, and Citrobacter rodentium are highly adapted enteropathogens that successfully colonize their host's gastrointestinal tract via the formation of attaching and effacing (A/E) lesions. These pathogens utilize a type III secretion system (TTSS) apparatus, encoded by the locus of enterocyte effacement, to translocate bacterial effector proteins into epithelial cells. Here, we report the identification of EspJ (E. coli-secreted protein J), a translocated TTSS effector that is carried on the 5' end of the cryptic prophage CP-933U. Infection of epithelial cells in culture revealed that EspJ is not required for A/E lesion activity in vivo and ex vivo. However, in vivo studies performed with mice demonstrated that EspJ possesses properties that influence the dynamics of clearance of the pathogen from the host's intestinal tract, suggesting a role in host survival and pathogen transmission.
引用
收藏
页码:679 / 686
页数:8
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