Furan is not genotoxic in the micronucleus assay in vivo or in vitro

被引:32
作者
Durling, Louise J. K. [1 ]
Svensson, Kettil [1 ]
Abramsson-Zetterberg, Lilianne [1 ]
机构
[1] Natl Food Adm Toxicol Lab, Div Toxicol, Livsmedelsverket, SE-75126 Uppsala, Sweden
关键词
furan; micronucleus assay; flow cytometer; chromosome aberration;
D O I
10.1016/j.toxlet.2006.08.020
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Furan, a potential human carcinogen, is formed during heat-treatment of food. Previous studies of the genotoxicity of furan have given disparate results. Hence, there is a need for complementary data to clarify the mechanism behind the carcinogenicity of furan. In this study, we have used the flow cytometer-based micronucleus assay in mice and the cytokinesis-block micronucleus assay in human lymphocytes to investigate the genotoxic potential of furan. Three in vivo experiments were performed: intraperitoneal or subcutaneous injection of furan in male Balb/C mice (0-300 and 0-275 mg/kg body weight, respectively) and intraperitoneal injection of male CBA mice (0 and 225 mg/kg body weight). No increased level of micronucleated erythrocytes was detected in any of the in vivo experiments. In the in vitro setup, human lymphocytes from two donors were treated with furan in concentrations from 0 to 100 mM, either with or without metabolic activation (liver homogenate from rat). In parity with the in vivo results there was no significant increase in the frequency of micronucleated cells here either. As neither the in vivo nor the in vitro studies disclose any significant increase in the micronucleus frequency after treatment with furan, our results support that the carcinogenicity of furan is caused by a non-genotoxic mechanism. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:43 / 50
页数:8
相关论文
共 36 条
[11]  
*EFSA, 2004, REP CONTAM PAN PROV
[12]  
*FDA, 2005, EXPL DAT FUR FOOD
[13]  
FDA (Food and Drug Administration), 2004, FUR FOOD THERM TREAT
[14]   MEASUREMENT OF MICRONUCLEI IN LYMPHOCYTES [J].
FENECH, M ;
MORLEY, AA .
MUTATION RESEARCH, 1985, 147 (1-2) :29-36
[15]   The in vitro micronucleus technique [J].
Fenech, M .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2000, 455 (1-2) :81-95
[16]   Furan-induced liver cell proliferation and apoptosis in female B6C3F1 mice [J].
FranssonSteen, R ;
Goldsworthy, TL ;
Kedderis, GL ;
Maronpot, RR .
TOXICOLOGY, 1997, 118 (2-3) :195-204
[17]   V79-hCYP2E1-hSULT1A1, a cell line for the sensitive detection of genotoxic effects induced by carbohydrate pyrolysis products and other food-borne chemicals [J].
Glatt, H ;
Schneider, H ;
Liu, YG .
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2005, 580 (1-2) :41-52
[18]   DNA CONTENT DETERMINATION OF MICRONUCLEATED POLYCHROMATIC ERYTHROCYTES INDUCED BY CLASTOGENS AND SPINDLE POISONS IN MOUSE BONE-MARROW AND PERIPHERAL-BLOOD [J].
GRAWE, J ;
ZETTERBERG, G ;
AMNEUS, H .
MUTAGENESIS, 1993, 8 (03) :249-255
[19]   FLOW-CYTOMETRIC ENUMERATION OF MICRONUCLEATED POLYCHROMATIC ERYTHROCYTES IN MOUSE PERIPHERAL-BLOOD [J].
GRAWE, J ;
ZETTERBERG, G ;
AMNEUS, H .
CYTOMETRY, 1992, 13 (07) :750-758
[20]   THE RELATIONSHIP BETWEEN DNA CONTENT AND CENTROMERE CONTENT IN MICRONUCLEATED MOUSE BONE-MARROW ERYTHROCYTES ANALYZED BY FLOW-CYTOMETRY AND FLUORESCENT IN-SITU HYBRIDIZATION [J].
GRAWE, J ;
ABRAMSSONZETTERBERG, L ;
ERIKSSON, L ;
ZETTERBERG, G .
MUTAGENESIS, 1994, 9 (01) :31-38