The self-assembly, characterization and application of hemoglobin immobilized on Fe3O4@Pt core-shell nanoparticles

被引：27

作者：

Fan, Hong ^{[1
]}

Pan, Zhong-Qin ^{[1
]}

Gu, Hai-Ying ^{[1
]}

机构：

[1] Nantong Univ, Sch Publ Hlth, Inst Analyt Chem Life Sci, Nantong 226007, Peoples R China

来源：

MICROCHIMICA ACTA | 2010年 / 168卷 / 3-4期

基金：

中国国家自然科学基金;

关键词：

Fe3O4@Pt core-shell nanoparticles; Hemoglobin; Hydrogen peroxide; Direct electron transfer; DIRECT ELECTRON-TRANSFER; GLASSY-CARBON ELECTRODE; DIRECT ELECTROCHEMISTRY; HORSERADISH-PEROXIDASE; GLUCOSE-OXIDASE; ELECTROCATALYSIS; NANOTUBES; MAGNETITE; SURFACE; MATRIX;

D O I：

10.1007/s00604-009-0279-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Direct electron transfer is demonstrated to occur between an electrode and hemoglobin that was immobilized on a film of Fe3O4@Pt-chitosan (Fe3O4@Pt-CS). Magnetic nanoparticles composed of Fe3O4 were prepared by a chemical coprecipitation method, and platinum nanoparticles were deposited on the Fe3O4 surface to form novel core-shell nanocomposites. In phosphate buffer solution of pH 7.0, the hemoglobin-Fe3O4@Pt-CS assembly on a modified glassy carbon electrode exhibited a couple of well-defined and quasi-reversible redox peaks. The formal potential E-0' was about -0.35 V. The electrode displayed excellent electrocatalytic activity towards oxygen and hydrogen peroxide reduction without the need for an electron mediator.

引用

页码：239 / 244

页数：6

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