Interaction of a cationic acrylate polymer with caseins: Biphasic effect of Eudragit E100 on the stability of casein micelles

被引:12
作者
Ausar, SF [1 ]
Bianco, ID [1 ]
Castagna, LF [1 ]
Alasino, RV [1 ]
Beltramo, DM [1 ]
机构
[1] Agencia Cordoba Ciencia, Ctr Excelencia Prod & Proc Cordoba, CEPROCOR, Cordoba, Argentina
关键词
Eudragit E100; casein micelles; Eudragit-casein interaction; aminoacryl methacrylate copolymer;
D O I
10.1021/jf034070b
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
When whole or skim milk was incubated with the cationic acrylate polymer Eudragit E100, a biphasic effect on the stability of casein micelles was observed. A precipitation phase was observed at low polymer/casein ratios. Strikingly, a solubilization phase of the aggregates was observed when the ratios of polymer/casein were increased. Purified alpha(s)-, beta-, and kappa-caseins or dephosphorylated caseins were equally precipitated and resolubilized by the cationic polymer, indicating no special selectivity for a particular protein or phosphate residue for these events. An increase in the size of the aggregates as the optimum precipitating amount of Eudragit E100 was reached suggests a crossbridging of the micelles by the polymer. The inhibition of the precipitation phase by high ionic strength indicates that electrostatic interactions play a critical role in complex formation. Furthermore, a dramatic reduction in size of the protein colloidal particles upon solubilization of the aggregates was observed by dynamic light scattering, indicating a dissociation of the micellar structure. Taken together, the results indicate that at low concentration Eudragit E100 may act as a precipitant of casein micelles, mainly by ionic interaction and at high concentration as an amphipathic agent, solubilizing casein micelles with a disruption of their internal structure.
引用
收藏
页码:4417 / 4423
页数:7
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