Neutrophil behavior in pancreas and liver and the role of nitric oxide in rat acute pancreatitis

被引:22
作者
Inagaki, H
Nakao, A
Kurokawa, T
Nonami, T
Harada, A
Takagi, H
机构
[1] Department of Surgery II, Nagoya University, School of Medicine, Showa-ku, Nagoya
[2] Department of Surgery II, Nagoya University, School of Medicine, Showa-ku, Nagoya 466
关键词
neutrophil; acute pancreatitis; rat; cerulein; nitric oxide;
D O I
10.1097/00006676-199710000-00014
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The behavior of neutrophils in a rat acute pancreatitis model was observed in the pancreas and liver using fluorescence microscopy with an image analyzing system after labeling with a specific fluorescent reagent. Nonviable cells of both organs were also labeled and quantified. The role of nitric oxide in neutrophil accumulation and organ damage was estimated by administering a relatively selective inhibitor of constitutive nitric oxide synthase, N-nitro-L-arginine (L-NNA). The animal model of acute pancreatitis was induced by cerulein injection (80 mg/kg). Two groups were created, one given and the other not given L-NNA (2.5 mg/kg) prior to the induction of pancreatitis. The number of accumulated neutrophils in the pancreas and liver increased in a time-dependent manner. There was a close relation between the distribution of the neutrophils and inviable acinar cells or hepatocytes. When pretreated with L-NNA, the numbers of accumulated neutrophils and nonviable cells increased significantly in the pancreas. In the liver, a more pronounced accumulation of neutrophils was observed after treatment with L-NNA. Although hepatocyte injury was mild despite the neutrophil accumulation in the control, such injury was marked in the group treated with L-NNA. This suggests that neutrophils serve an important role in exacerbating acute pancreatitis and that nitric oxide provides a defense mechanism against neutrophil accumulation in pancreas and liver.
引用
收藏
页码:304 / 309
页数:6
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