Identification of three mouse μ-opioid receptor (MOR) gene (Oprm1) splice variants containing a newly identified alternatively spliced exon

被引:25
作者
Doyle, Glenn A. [1 ]
Sheng, X. Rebecca [1 ]
Lin, Sharon S. J. [1 ]
Press, Danielle M. [1 ]
Grice, Dorothy E. [1 ]
Buono, Russell J. [1 ]
Ferraro, Thomas N. [1 ]
Berrettini, Wade H. [1 ]
机构
[1] Univ Penn, Dept Psychiat, Ctr Neurobiol & Behav, Philadelphia, PA 19104 USA
关键词
murine; opiate receptor; C57BL/6; DBA/2; cDNA; polymorphism;
D O I
10.1016/j.gene.2006.10.017
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The mouse It-opioid receptor gene, Oprm 1, is recognized currently to contain 17 alternatively spliced exons that generate 24 splice variants encoding at least I I morphine-binding isoforms of the receptor. Here, we identify three new MOR splice variants that contain a previously undescribed exon, exon 18, and provide evidence that they are expressed in two mouse strains. The transcripts containing the newly identified exon 18 encode two new putative mu-opioid receptor isofonns, MOR-1V and MOR-1W. In mouse Oprm1, exon 18 is located between the described exons 10 and 6. Exon 18 appears to be conserved in the rat genome between exons 4 and 7. A BLAST search of the non-redundant GenBank database suggests that human OPRM1 may also contain exon 18. Analysis of mouse brain mRNA by RT-PCR suggests that MOR-1Vii transcripts are expressed in all areas of the brain analyzed, whereas expression of MOR-1Vi transcripts was restricted to thalamus and striatum. MOR-1W transcripts are expressed most highly in the hypothalamus, thalamus and striatum. In summary, we have identified three brain expressed, alternatively spliced mouse MOR splice variants containing a novel exon and encoding new putative MOR isoforms, MOR-1V and MOR-1W. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:135 / 147
页数:13
相关论文
共 59 条
[1]   Comparative immunohistochemical distributions of carboxy terminus epitopes from the mu-opioid receptor splice variants MOR-1D, MOR-1 and MOR-1C in the mouse and rat CNS [J].
Abbadie, C ;
Pan, YX ;
Drake, CT ;
Pasternak, GW .
NEUROSCIENCE, 2000, 100 (01) :141-153
[2]   Differential in vivo internalization of MOR-1 and MOR-1C by morphine [J].
Abbadie, C ;
Pasternak, GW .
NEUROREPORT, 2001, 12 (14) :3069-3072
[3]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[4]   Functional magnetic resonance imaging measures of the effects of morphine on central nervous system circuitry in opioid-naive healthy volunteers [J].
Becerra, Lino ;
Harter, Kim ;
Gonzalez, R. Gilberto ;
Borsook, David .
ANESTHESIA AND ANALGESIA, 2006, 103 (01) :208-216
[5]   VOLUNTARY CONSUMPTION OF MORPHINE IN 15 INBRED MOUSE STRAINS [J].
BELKNAP, JK ;
CRABBE, JC ;
RIGGAN, J ;
OTOOLE, LA .
PSYCHOPHARMACOLOGY, 1993, 112 (2-3) :352-358
[6]   QUANTITATIVE TRAIT LOCI MAPPING OF 3 LOCI CONTROLLING MORPHINE PREFERENCE USING INBRED MOUSE STRAINS [J].
BERRETTINI, WH ;
FERRARO, TN ;
ALEXANDER, RC ;
BUCHBERG, AM ;
VOGEL, WH .
NATURE GENETICS, 1994, 7 (01) :54-58
[7]   Sequence and structure-based prediction of eukaryotic protein phosphorylation sites [J].
Blom, N ;
Gammeltoft, S ;
Brunak, S .
JOURNAL OF MOLECULAR BIOLOGY, 1999, 294 (05) :1351-1362
[8]   Functional analysis of MOR-1 splice variants of the mouse mu opioid receptor gene Oprm [J].
Bolan, EA ;
Pan, YX ;
Pasternak, GW .
SYNAPSE, 2004, 51 (01) :11-18
[9]   Decreased stability and translation of T cell receptor ζ mRNA with an alternatively spliced 3′-untranslated region contribute to ζ chain down-regulation in patients with systemic lupus erythematosus [J].
Chowdhury, B ;
Tsokos, CG ;
Krishnan, S ;
Robertson, J ;
Fisher, CU ;
Warke, RG ;
Warke, VG ;
Nambiar, MP ;
Tsokos, GC .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (19) :18959-18966
[10]   Endocytosis of G protein-coupled receptors:: roles of G protein-coupled receptor kinases and β-arrestin proteins [J].
Claing, A ;
Laporte, SA ;
Caron, MG ;
Lefkowitz, RJ .
PROGRESS IN NEUROBIOLOGY, 2002, 66 (02) :61-79