共 59 条
Identification of three mouse μ-opioid receptor (MOR) gene (Oprm1) splice variants containing a newly identified alternatively spliced exon
被引:25
作者:
Doyle, Glenn A.
[1
]
Sheng, X. Rebecca
[1
]
Lin, Sharon S. J.
[1
]
Press, Danielle M.
[1
]
Grice, Dorothy E.
[1
]
Buono, Russell J.
[1
]
Ferraro, Thomas N.
[1
]
Berrettini, Wade H.
[1
]
机构:
[1] Univ Penn, Dept Psychiat, Ctr Neurobiol & Behav, Philadelphia, PA 19104 USA
来源:
关键词:
murine;
opiate receptor;
C57BL/6;
DBA/2;
cDNA;
polymorphism;
D O I:
10.1016/j.gene.2006.10.017
中图分类号:
Q3 [遗传学];
学科分类号:
071007 ;
090102 ;
摘要:
The mouse It-opioid receptor gene, Oprm 1, is recognized currently to contain 17 alternatively spliced exons that generate 24 splice variants encoding at least I I morphine-binding isoforms of the receptor. Here, we identify three new MOR splice variants that contain a previously undescribed exon, exon 18, and provide evidence that they are expressed in two mouse strains. The transcripts containing the newly identified exon 18 encode two new putative mu-opioid receptor isofonns, MOR-1V and MOR-1W. In mouse Oprm1, exon 18 is located between the described exons 10 and 6. Exon 18 appears to be conserved in the rat genome between exons 4 and 7. A BLAST search of the non-redundant GenBank database suggests that human OPRM1 may also contain exon 18. Analysis of mouse brain mRNA by RT-PCR suggests that MOR-1Vii transcripts are expressed in all areas of the brain analyzed, whereas expression of MOR-1Vi transcripts was restricted to thalamus and striatum. MOR-1W transcripts are expressed most highly in the hypothalamus, thalamus and striatum. In summary, we have identified three brain expressed, alternatively spliced mouse MOR splice variants containing a novel exon and encoding new putative MOR isoforms, MOR-1V and MOR-1W. (c) 2006 Elsevier B.V. All rights reserved.
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页码:135 / 147
页数:13
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