Size-dependent free solution DNA electrophoresis in structured microfluidic systems

被引：47

作者：

Duong, TT

Kim, G

Ros, R

Streek, M

Schmid, F

Brugger, J

Anselmetti, D

Ros, A ^{[1
]}

机构：

[1] Univ Bielefeld, Dept Phys, D-4800 Bielefeld, Germany

[2] Ecole Polytech Fed Lausanne, Microsyst Lab, Lausanne, Switzerland

来源：

MICROELECTRONIC ENGINEERING | 2003年 / 67-8卷

关键词：

DNA; electrophoresis; microchip; contact lithography; single molecule; fluorescence microscopy;

D O I：

10.1016/S0167-9317(03)00153-9

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

A new concept based on microstructuring techniques is presented to achieve size-dependent electrophoretic migration of DNA in free solution. Topographically structured microfluidic channels with periodical cavities in the range of the radius of gyration of the tested DNA molecules (< 3 mum) are produced by moulding of polydimethylsiloxane on a master wafer fabricated by contact lithography of SU-8 photoresist. The electrophoretic migration of single DNA molecules stained with the intercalator YOYO was investigated by real-time fluorescence video microscopy. It could be demonstrated for large DNA molecules that lambda-DNA (48 kbp) always migrated significantly faster than T2-DNA (164 kbp) in the two tested microchannel layouts. Integration into a microdevice for DNA separation in free solution is now in progress. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：905 / 912

页数：8

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