Construction of the expression vector based on the growth phase- and growth rate-dependent rmf promoter:: use of cell growth rate to control the expression of cloned genes in Escherichia coli

被引：6

作者：

Chao, YP

Wen, CS

Chiang, CJ

Wang, JJ

机构：

[1] Feng Chia Univ, Dept Chem Engn, Taichung 40724, Taiwan

[2] Widetex Chem Co, Taipei, Taiwan

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 01期

关键词：

bioremediation; expression vector; growth phase; ribosome modulation factor;

D O I：

10.1023/A:1026775906596

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel expression vector based on the rmf promoter (Wada et al. Proc. Natl. Acad. Sci. USA (1995) 87: 2657-2661) was developed. Using lacZ gene as a model, protein production depending on cell growth phase and growth rate was found. In addition, by subjecting the recombinant cells to temperature downshift and substrate famine, the strain was capable of producing 35 000-40 000 units of beta -galactosidase. These characteristics indicate the potential application of this expression vector to bioprocessing such as protein production in continuous or batch fermentation, biotransformation using entrapped cells, and in situ bioremediation.

引用

页码：5 / 11

页数：7