Packing columns for capillary electrochromatography

被引:90
作者
Colón, LA [1 ]
Maloney, TD [1 ]
Fermier, AM [1 ]
机构
[1] SUNY Buffalo, Dept Chem, Buffalo, NY 14260 USA
基金
美国国家科学基金会;
关键词
reviews; stationary phases; CEC; electrochromatography; packed columns; packing procedures;
D O I
10.1016/S0021-9673(99)01328-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Considering the current interest in capillary electrochromatography (CEC), performed in packed columns, we present the different methods used to pack capillary columns for use in CEC. General considerations on column packing are given and the column fabrication process is discussed in sufficient detail to allow instruction to those who are not experienced in the field. Five different packing methods are discussed to deliver packing material into the capillary column from a practical view point: slurry pressure packing, packing with supercritical CO2, electrokinetic packing, using centripetal forces, and packing by gravity. Entrapment of particulate material by sintering and sol-gel technology is also mentioned. Although slurry pressure packing procedures are most common, higher separation efficiencies are obtained using other packing approaches. Electrokinetic packing seems to be the simplest technique to deliver the packing material into the capillary columns. Nevertheless, as with the other packing techniques, skill and experience are required to complete all the steps involved in the fabrication of packed columns for CEC. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:43 / 53
页数:11
相关论文
共 79 条
  • [61] CAPILLARY ELECTROCHROMATOGRAPHY ELECTROSPRAY MASS-SPECTROMETRY - A MICROANALYSIS TECHNIQUE
    SCHMEER, K
    BEHNKE, B
    BAYER, E
    [J]. ANALYTICAL CHEMISTRY, 1995, 67 (20) : 3656 - 3658
  • [62] High performance capillary gel electrochromatography with replaceable media
    Schure, MR
    Murphy, RE
    Klotz, WL
    Lau, W
    [J]. ANALYTICAL CHEMISTRY, 1998, 70 (23) : 4985 - 4995
  • [63] Molecular imprinting for chiral separations and drug screening purposes using monolithic stationary phases in CEC
    Schweitz, L
    Andersson, LI
    Nilsson, S
    [J]. CHROMATOGRAPHIA, 1999, 49 (Suppl 1) : S93 - S94
  • [64] Capillary electrochromatography with predetermined selectivity obtained through molecular imprinting
    Schweitz, L
    Andersson, LI
    Nilsson, S
    [J]. ANALYTICAL CHEMISTRY, 1997, 69 (06) : 1179 - 1183
  • [65] Capillary electrochromatography with 1.8-μm ODS-modified porous silica particles
    Seifar, RM
    Kraak, JC
    Kok, WT
    Poppe, H
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1998, 808 (1-2) : 71 - 77
  • [66] THE ANALYSIS OF PHARMACEUTICAL COMPOUNDS USING ELECTROCHROMATOGRAPHY
    SMITH, NW
    EVANS, MB
    [J]. CHROMATOGRAPHIA, 1994, 38 (9-10) : 649 - 657
  • [67] Monolithic columns containing sol-gel bonded octadecylsilica for capillary electrochromatography
    Tang, QL
    Xin, BM
    Lee, ML
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1999, 837 (1-2) : 35 - 50
  • [68] TSUDA T, 1982, J CHROMATOGR, V248, P241, DOI 10.1016/S0021-9673(00)87275-1
  • [69] Experiences with packed capillary electrochromatography at ambient pressure
    vandenBosch, SE
    Heemstra, S
    Kraak, JC
    Poppe, H
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1996, 755 (02) : 165 - 177
  • [70] PSEUDO-ELECTROCHROMATOGRAPHY MASS-SPECTROMETRY - A NEW ALTERNATIVE
    VERHEIJ, ER
    TJADEN, UR
    NIESSEN, WMA
    VANDERGREEF, J
    [J]. JOURNAL OF CHROMATOGRAPHY, 1991, 554 (1-2): : 339 - 349