Isolation of microsatellite markers in Cannabis sativa L. (marijuana)

被引:42
作者
Gilmore, S
Peakall, R
机构
[1] Canberra Inst Technol, Ctr Forens Sci, Canberra, ACT 2601, Australia
[2] Australian Natl Univ, Sch Bot & Zool, Canberra, ACT 0200, Australia
来源
MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 01期
关键词
Cannabis; forensic; germplasm; hemp; microsatellites;
D O I
10.1046/j.1471-8286.2003.00367.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have identified 15 variable microsatellite loci in Cannabis sativa. In 48 samples from five fibre crop seed accessions, we detected an average of 10 alleles per locus (range 2-28) with mean heterozygosity of 0.68 (range 0.28-0.94). Significant genetic differentiation was found between accessions (F-ST = 0.12, P < 0.001). These markers have utility for characterizing genetic diversity in cultivated and naturalized Cannabis populations.
引用
收藏
页码:105 / 107
页数:3
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