Outstanding stability of immobilized recombinant α(1→3/4)-fucosyltransferases exploited in the synthesis of Lewis a and Lewis x trisaccharides

被引：18

作者：

Augé, C ^{[1
]}

Malleron, A

Tahrat, H

Marc, A

Goergen, JL

Cerutti, M

Steelant, WFA

Delannoy, P

Lubineau, A

机构：

[1] Univ Paris Sud, Lab Chim Organ Multifonctionnelle, UMR 8614, F-91405 Orsay, France

[2] INPL, CNRS, Lab Sci Genie Chim, F-54505 Vandoeuvre Nancy, France

[3] INRA, Lab Pathol Comparee, URA 2209, Unite Biol Cellulaire & Mol, F-30380 St Christol Lez Ales, France

[4] INRA, UPR 27, F-30380 St Christol Lez Ales, France

[5] Univ Sci & Tech Lille Flandres Artois, Chim Biol Lab, UMR 8576, Unite Glycobiol Struct & Fonctionnelle, F-59655 Villeneuve Dascq, France

来源：

CHEMICAL COMMUNICATIONS | 2000年 / 20期

关键词：

D O I：

10.1039/b006063l

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Recombinant human alpha(1 --> 3/4)-fucosyltransferases (FucT-III) expressed in CHO cells or baculovirus-infected insect cells, immobilized on Ni2+-agarose through a 6His tag, exhibit a marked stability, which was exploited in the synthesis of Lewis a and Lewis x trisaccharides.

引用

页码：2017 / 2018

页数：2

共 8 条

[1] Enzymatic synthesis of sialyl-Lewis-libraries with two nonnatural monosaccharide units [J].

Baisch, G ;

Öhrlein, R ;

Streiff, M ;

Kolbinger, F .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1998, 8 (07) :755-758

[2]

Benslimane C, 1999, ANIMAL CELL TECHNOLOGY: PRODUCTS FROM CELLS, CELLS AS PRODUCTS, P251

[3]

Costa J, 1997, J BIOL CHEM, V272, P11613

[4] ACCEPTOR SPECIFICITY OF DIFFERENT LENGTH CONSTRUCTS OF HUMAN RECOMBINANT ALPHA-1,3/4-FUCOSYL-TRANSFERASES - REPLACEMENT OF THE STEM REGION AND THE TRANSMEMBRANE DOMAIN OF FUCOSYL-TRANSFERASE-V BY PROTEIN-A RESULTS IN AN ENZYME WITH GDP-FUCOSE HYDROLYZING ACTIVITY [J].

DEVRIES, T ;

SRNKA, CA ;

PALCIC, MM ;

SWIEDLER, SJ ;

VANDENEIJNDEN, DH ;

MACHER, BA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (15) :8712-8722

[5] Heterologous expression of an engineered truncated form of human Lewis fucosyltransferase (Fuc-TIII) by the methylotrophic yeast Pichia pastoris [J].

Gallet, PF ;

Vaujour, H ;

Petit, JM ;

Maftah, A ;

Oulmouden, A ;

Oriol, R ;

Le Narvor, C ;

Guilloton, M ;

Julien, R .

GLYCOBIOLOGY, 1998, 8 (09) :919-925

[6] NEW METAL CHELATE ADSORBENT SELECTIVE FOR PROTEINS AND PEPTIDES CONTAINING NEIGHBORING HISTIDINE-RESIDUES [J].

HOCHULI, E ;

DOBELI, H ;

SCHACHER, A .

JOURNAL OF CHROMATOGRAPHY, 1987, 411 :177-184

[7] A CLONED HUMAN CDNA DETERMINES EXPRESSION OF A MOUSE STAGE-SPECIFIC EMBRYONIC ANTIGEN AND THE LEWIS BLOOD GROUP-ALPHA-(1,3/1,4)FUCOSYLTRANSFERASE [J].

KUKOWSKALATALLO, JF ;

LARSEN, RD ;

NAIR, RP ;

LOWE, JB .

GENES & DEVELOPMENT, 1990, 4 (08) :1288-1303

[8] The chemoenzymatic synthesis of the core trisaccharide of N-linked oligosaccharides using a recombinant β-mannosyltransferase [J].

Watt, GM ;

Revers, L ;

Webberley, MC ;

Wilson, IBH ;

Flitsch, SL .

CARBOHYDRATE RESEARCH, 1997, 305 (3-4) :533-541

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