A role for β-actin in RNA polymerase III transcription

被引：172

作者：

Hu, P

Wu, S

Hernandez, N

机构：

[1] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

[2] Cold Spring Harbor Lab, Howard Hughes Med Inst, Cold Spring Harbor, NY 11724 USA

来源：

GENES & DEVELOPMENT | 2004年 / 18卷 / 24期

关键词：

RNA polymerase III; beta-actin; human U6 snRNA promoter; CK2;

D O I：

10.1101/gad.1250804

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

When transcription from the human U6 snRNA gene is reconstituted with recombinant factors and purified RNA polymerase III (pol III), pol III must be treated with CK2 to be active. We show that highly purified pol III contains associated beta-actin, and beta-actin localizes to an active U6 promoter in vivo. Pol III immunoprecipitated from IMR90 cells treated with a genotoxic agent lacks associated beta-actin and is inactive in the reconstituted assay. Transcription is regained upon treatment of pol III with CK2 and addition of beta-actin. This suggests that beta-actin associated with pol III is essential for basal pol III transcription.

引用

收藏

页码：3010 / 3015

页数：6

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