A protein chip approach for high-throughput antigen identification and characterization

被引：19

作者：

Hu, Shaohui

Li, Yu

Liu, Guozhen

Song, Qifeng

Wang, Li

Han, Yuning

Zhang, Yang

Song, Yali

Yao, Xiying

Tao, Yong

Zeng, Haipan

Yang, Huanming

Wang, Jian

Zhu, Heng

Chen, Zhi-Nan

Wu, Lin

机构：

[1] Chinese Acad Sci, Beijing Inst Genom, Beijing 101318, Peoples R China

[2] Fourth Mil Med Univ, Dept Cell Biol, Cell Engn Res Ctr, Xian, Shanxi, Peoples R China

[3] Agr Univ Hebei, Coll Life Sci, Baoding, Hebei, Peoples R China

[4] Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD USA

[5] Johns Hopkins Univ, Sch Med, HiT Ctr, Baltimore, MD USA

来源：

PROTEOMICS | 2007年 / 7卷 / 13期

关键词：

biomarker; liver proteins; monoclonal antibody; protein microarray;

D O I：

10.1002/pmic.200600923

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteomics research in humans and other eukaryotes demands a large number of high-quality mAbs. Here, we report a new approach to produce high-quality mAbs against human liver proteins using a combined force of high-throughput mAb production and protein microarrays. After immunizing mice with live cells from human livers, we isolated 54 hybridomas with binding activities to human cells and identified the corresponding antigens for five mAbs via screening on a protein microarray of 1.058 unique human liver proteins. Finally, we demonstrated that using the five mAbs we could characterize the expression profiles of their corresponding antigens by using tissue microarrays. Among them, we discovered that eIF1A expressed only in normal liver tissues, not in hepatocellular carcinoma in humans.

引用

页码：2151 / 2161

页数：11

共 31 条

[11] Human Liver Proteome Project - Plan, progress, and perspectives [J].