A novel alpha-helical protein, specific to and highly conserved in plants, is associated with the nuclear matrix fraction

被引：13

作者：

Rose, A

Gindullis, F

Meier, I ^{[1
]}

机构：

[1] Ohio State Univ, Ctr Plant Biotechnol, Columbus, OH 43210 USA

[2] Ohio State Univ, Dept Plant Biol, Columbus, OH 43210 USA

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 2003年 / 54卷 / 385期

基金：

美国国家科学基金会;

关键词：

amphipathic alpha helix; GFP; nuclear matrix; tomato;

D O I：

10.1093/jxb/erg114

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A cDNA for a novel plant protein was isolated from tomato. Nuclear Matrix Protein 1 (NMP1) is a ubiquitously expressed 36 kDa protein, which has no homologues in animals and fungi, but is highly conserved among flowering and non-flowering plants, including gymnosperms, moss, and the liverwort Marchantia polymorpha. NMP1 is predominantly a-helical with multiple stretches of short amphipathic regions. Cell fractionation, immunofluorescence, and GFP localization experiments showed that NMP1 is located both in the cytoplasm and nucleus and that the nuclear fraction is associated with the nuclear matrix. NMP1 is a candidate for a plant-specific structural protein with a function both in the nucleus and cytoplasm.

引用

页码：1133 / 1141

页数：9

共 44 条

[31] LPP, an actin cytoskeleton protein related to zyxin, harbors a nuclear export signal and transcriptional activation capacity [J].