A comparison between the use of a redox mediator in solution and of surface modified electrodes in the electrocatalytic oxidation of nicotinamide adenine dinucleotide

被引：20

作者：

Antiochia, R ^{[1
]}

Lavagnini, I ^{[1
]}

Pastore, P ^{[1
]}

Magno, F ^{[1
]}

机构：

[1] Univ Padua, Dipartimento Chim Inorgan Met & Anal, I-35131 Padua, Italy

来源：

BIOELECTROCHEMISTRY | 2004年 / 64卷 / 02期

关键词：

NADH oxidation; cyclic voltammetry; p-methylamino-phenolsulphate; 3,4-dihydroxybenzaldehyde; modified electrode; carbon nanotube paste electrode;

D O I：

10.1016/j.bioelechem.2004.01.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cyclic voltammetry was successfully applied to study the oxidation of nicotinamide adenine dinucleotide (NADH) both in homogeneous and heterogeneous phase. The first case was realized with a solution containing p-methylamino-phenolsulphate (MAP) as redox mediator and the diaphorase (DI) from Clostridium kluveri as enzyme while the second one by using both a glassy carbon (GC) and a carbon nanotube paste (CNTP) electrode modified with electrodeposited films derived from 3,4-dihydroxybenzaldehyde (3,4-DHB). Such systems were successively coupled with glucose dehydrogenase (GDH) reaction to realize the redox chain present in glucose biosensors. A critical comparison of the two systems was also reported. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：157 / 163

页数：7

共 37 条

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