16S rDNA sequence analysis of Xylella fastidiosa strains

被引：11

作者：

Chen, J ^{[1
]}

Jarret, RL

Qin, X

Hartung, JS

Banks, D

Chang, CJ

Hopkins, DL

机构：

[1] Florida A&M Univ, Ctr Viticultural Sci, Tallahassee, FL 32307 USA

[2] USDA ARS, Dept Plant Genet Resources, Griffin, GA USA

[3] USDA ARS, Fruit Lab, Beltsville Agr Res Ctr, Beltsville, MD USA

[4] Univ Georgia, Dept Plant Pathol, Griffin, GA USA

[5] Univ Florida, Cent Florida Res & Educ Ctr, Apopka, FL USA

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2000年 / 23卷 / 03期

关键词：

16S rDNA; Pierce's disease; taxonomy; sequence analysis; Xylella fastidiosa;

D O I：

10.1016/S0723-2020(00)80064-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The 16S rDNA encoding the small subunit ribosomal RNA were amplified by PCR, cloned, and sequenced from 16 strains of Xylella fastidiosa originating from nine different hosts. In pair-wise comparisons, X. fastidiosa strains showed a maximum variation of 1.0% or 14 nucleotide positions. When all 16 sequences were considered as a set, 54 variable positions were found. Analysis of the sequence data indicated that the X. fastdiosa strains formed three rDNA groups. Group one includes Pierce's disease and mulberry leaf scorch strains; Group two, periwinkle wilt, plum leaf scald, phony peach, oak leaf scorch, and elm leaf scorch strains; and Group three, citrus variegated chlorosis and coffee leaf scorch strains. All X. fastidiosa strains exhibited significantly higher levels of sequence heterogeneity (63 to 83 nucleotide positions) when compared to species from Xanthomonas and Stenotrophomonas. Our data demonstrate that 16S rDNA sequence data could provide valuable information for future classification of X. fastidiosa at the sub-species level.

引用

页码：349 / 354

页数：6

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