Development of a 5′-nuclease PCR assay for detecting Shiga toxin-producing Escherichia coli O145 based on the identification of an 'O-island 29' homologue

被引:12
作者
Perelle, S [1 ]
Dilasser, F [1 ]
Grout, J [1 ]
Fach, P [1 ]
机构
[1] AFSSA, Lab Etud & Rech Hyg & Qual Aliments, Unite Atelier Biotechnol, F-94700 Maisons Alfort, France
关键词
'O-island 29'; real-time PCR; STEC O145;
D O I
10.1046/j.1365-2672.2003.01872.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: A DNA sequence, from Escherichia coli STEC O145, homologous to O-island 29 from STEC O157 is described, together with a real-time PCR assay for detecting it. Methods and Results: PCR and sequencing were used to identify the 'O-island 29' homologous DNA sequence from STEC O145 (strain VTH34). The sequence divergence between the STEC O145 and O157 'O-island 29' allowed a STEC O145 5'-nuclease PCR assay to be developed. Conclusions: The characterization of a novel locus in STEC O145 has allowed a specific O145 serogroup 5'-nuclease PCR assay to be designed. Significance and Impact of the Study: These findings increase the number of serogroup PCR assays available as alternatives to classical O-serotyping of E. coli .
引用
收藏
页码:587 / 594
页数:8
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