The human homolog of rat Jagged1 expressed by marrow stroma inhibits differentiation of 32D cells through interaction with Notch1

被引：231

作者：

Li, LH

Milner, LA

Deng, Y

Iwata, M

Banta, A

Graf, L

Marcovina, S

Friedman, C

Trask, BJ

Hood, L ^{[1
]}

Torok-Storb, B

机构：

[1] Univ Washington, Stower Inst Med Res, Seattle, WA 98195 USA

[2] Univ Washington, Dept Mol Biotechnol, Seattle, WA 98195 USA

[3] Univ Washington, NW Lipid Res Lab, Seattle, WA 98195 USA

[4] Fred Hutchinson Canc Res Ctr, Div Clin Res, Seattle, WA 98104 USA

[5] Fudan Univ, Inst Genet, Shanghai 200433, Peoples R China

来源：

IMMUNITY | 1998年 / 8卷 / 01期

关键词：

D O I：

10.1016/S1074-7613(00)80457-4

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A cDNA clone encoding the human homolog of rat Jagged1 was isolated from normal human marrow. Analyses of human stromal cell lines indicate that this gene, designated hJagged1, is expressed by marrow stromal cells typified by the cell line HS-27a, which supports the long-term maintenance of hematopoietic progenitor cells. G-CSF-induced differentiation of 32D cells expressing Notch1 was inhibited by coculturing with HS-27a. A peptide corresponding to the Delta/Serrate/LAG-2 domain of hJagged1 and supernatants from COS cells expressing a soluble form of the extracellular portion of hJagged1 were able to mimic this effect. These observations suggest that hJagged1 may function as a ligand for Notch1 and play a role in mediating cell fate decisions during hematopoiesis.

引用

页码：43 / 55

页数：13

共 62 条

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