Blue light regeneration of bacteriorhodopsin bleached by continuous light

被引:12
作者
Dancsházy, Z [1 ]
Tokaji, Z [1 ]
机构
[1] Hungarian Acad Sci, Biol Res Ctr, Inst Biophys, H-6701 Szeged, Hungary
基金
匈牙利科学研究基金会;
关键词
blue light effect; stability in vivo; technical application of bacteriorhodopsin; photoreaction; thermal denaturation;
D O I
10.1016/S0014-5793(00)01714-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Photobleaching of bacteriorhodopsin (BR) by continuous light has recently been demonstrated. This bleaching consists of at least two subsequent product states. One of them is absorbing maximally in the blue spectral region. Our present study shows that upon illumination of the bleached sample with blue light a back photoprocess appears, resulting in regeneration of the original BR state. From a technical point of view, the observed phenomenon is similar to the reverting effect of blue light on the photocycle. An important difference is that the photobleached state of BR is much more stable than any of the photocycle intermediates, and may provide an advantage for several technical applications. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:171 / 173
页数:3
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